{"id":"W4414169467","doi":"10.1002/cyto.b.22253","title":"Ad hoc antibody modification of a validated flow cytometric immunophenotyping panel—recommendations and safeguards for clinical laboratories","year":2025,"lang":"en","type":"article","venue":"Cytometry Part B Clinical Cytometry","topic":"Single-cell and spatial transcriptomics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"London Health Sciences Centre","funders":"","keywords":"Immunophenotyping; Documentation; Protocol (science); Troubleshooting; Flow cytometry; Antibody; Standard operating procedure","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.003050957,0.0003852009,0.0009614825,0.00083229,0.0002721419,0.0000951006,0.0005170291,0.0007947434,0.00002878929],"category_scores_gemma":[0.005473826,0.0003890987,0.0004683337,0.003351294,0.0005371631,0.00003019871,0.0002344704,0.0004517453,0.00000806129],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003527545,"about_ca_system_score_gemma":0.0003372006,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005132438,"about_ca_topic_score_gemma":0.000004035302,"domain_scores_codex":[0.9956815,0.0004402931,0.002122875,0.001007794,0.0002616799,0.0004858386],"domain_scores_gemma":[0.9962175,0.001311057,0.000533146,0.0009048831,0.0008081736,0.0002252012],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"observational","study_design_gemma":"observational","study_design_scores_codex":[0.003054145,0.002760491,0.532518,0.0006923944,0.001725447,0.000003178509,0.00006820946,0.00002719875,0.2676692,0.0005091258,0.008010794,0.1829618],"study_design_scores_gemma":[0.01588281,0.004721224,0.4399448,0.0006682325,0.001206793,0.00001344737,0.0006406886,0.002911604,0.1598354,0.0007392201,0.3713767,0.002059073],"study_design_candidate":"observational","study_design_consensus":"observational","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8808751,0.009429567,0.1052075,0.0004354477,0.002331558,0.0007370394,0.000657465,0.00007028662,0.0002559963],"genre_scores_gemma":[0.969677,0.01451654,0.0128973,0.0005258279,0.0004514339,0.00006164363,0.001439806,0.00005459211,0.0003758176],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.3633659,"threshold_uncertainty_score":0.9998561,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1153037241675442,"score_gpt":0.442862785359713,"score_spread":0.3275590611921687,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}