{"id":"W4414212063","doi":"10.12688/f1000research.169502.1","title":"A guide to selecting high-performing antibodies for VCP (UniProt ID: P55072) for use in western blot, immunoprecipitation, and immunofluorescence","year":2025,"lang":"en","type":"preprint","venue":"F1000Research","topic":"Endoplasmic Reticulum Stress and Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; Montreal Neurological Institute and Hospital","funders":"Québec Consortium for Drug Discovery; Mitacs; ALS Society of Canada; Motor Neurone Disease Association; ALS Association","keywords":"Antibody; Immunofluorescence; Open peer review; Plant biology; Disease; Protocol (science); Organelle; ATPase","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0007118899,0.0003169096,0.0003736561,0.0003116379,0.0002312931,0.0002600717,0.0005004811,0.000297353,0.000004407136],"category_scores_gemma":[0.001475009,0.0003255203,0.000114922,0.0001528086,0.00009917105,0.00001483134,0.001244059,0.0002969424,0.000001524559],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006697563,"about_ca_system_score_gemma":0.0004819401,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001709445,"about_ca_topic_score_gemma":0.0007565285,"domain_scores_codex":[0.9976577,0.0001043167,0.000482982,0.0008877749,0.0002410922,0.0006262019],"domain_scores_gemma":[0.9982669,0.0003057036,0.0001062126,0.0006037824,0.0005736542,0.0001437479],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.007500071,0.0005943119,0.1757181,0.01074069,0.0009887073,0.00003334798,0.002491825,0.005107152,0.7001593,0.001254233,0.0322377,0.06317464],"study_design_scores_gemma":[0.01233666,0.002909243,0.07734384,0.006251047,0.0003481222,0.0000304234,0.002277946,0.01239554,0.776108,0.003622036,0.1026785,0.003698592],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9833787,0.002250454,0.009261658,0.0003439977,0.000240351,0.003631442,0.0007530425,0.00002450312,0.0001158514],"genre_scores_gemma":[0.9345541,0.002377243,0.04551439,0.0002541716,0.0003913609,0.004853611,0.001957464,0.0001031748,0.009994493],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.09837421,"threshold_uncertainty_score":0.9999197,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02489793237594751,"score_gpt":0.3597203619218893,"score_spread":0.3348224295459418,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}