{"id":"W4414356495","doi":"10.3390/antib14030080","title":"Development of a Chicken Immunoglobulin Heavy Chain Variable Region (VH) Single-Domain Antibody (sdAb) Against Calsequestrin (CSQ) and Its Application","year":2025,"lang":"en","type":"article","venue":"Antibodies","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Aging","funders":"","keywords":"Fusion protein; Immunoglobulin light chain; Recombinant DNA; Antibody; Phage display; Protein engineering; Single-chain variable fragment; Alexa Fluor; Protein G; Protein A/G","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003221606,0.0004705489,0.0001655201,0.0003156719,0.0001169329,0.0002233797,0.0003294349,0.0004420356,0.0006318065],"category_scores_gemma":[0.0002497728,0.0001672831,0.0003101779,0.0001699717,0.0001570499,0.0001840378,0.0002639392,0.0005309261,0.0004111162],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003299032,"about_ca_system_score_gemma":0.0002300816,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000743271,"about_ca_topic_score_gemma":0.0007841961,"domain_scores_codex":[0.9997875,0.00002939868,0.00001786065,0.000055909,0.00007434587,0.00003492898],"domain_scores_gemma":[0.9998184,0.00002654692,0.00002644701,0.00001695245,0.00007569823,0.00003600138],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001361141,0.0000148785,0.0002508033,0.00002698913,0.000003274384,0.00002258239,0.000008358905,0.0000721892,0.9970912,0.00004370452,0.00002749749,0.00242482],"study_design_scores_gemma":[0.000010628,0.0002910865,0.002212834,0.000009097671,0.0000214509,0.000488,0.00002186906,0.002154565,0.9912442,0.00003674083,0.003504932,0.000004489513],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9170129,0.002016153,0.07807981,0.0002566271,0.00005859117,0.0002137194,0.0002898075,0.0001593569,0.001912944],"genre_scores_gemma":[0.941677,0.001313627,0.05294712,0.0001970842,0.00001739278,0.00008420561,0.001186696,0.00002572259,0.002551189],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000743271,"threshold_uncertainty_score":0.002393603,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01279473261424524,"score_gpt":0.251205336855404,"score_spread":0.2384106042411588,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}