{"id":"W4414356495","doi":"10.3390/antib14030080","title":"Development of a Chicken Immunoglobulin Heavy Chain Variable Region (VH) Single-Domain Antibody (sdAb) Against Calsequestrin (CSQ) and Its Application","year":2025,"lang":"en","type":"article","venue":"Antibodies","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Aging","funders":"","keywords":"Fusion protein; Immunoglobulin light chain; Recombinant DNA; Antibody; Phage display; Protein engineering; Single-chain variable fragment; Alexa Fluor; Protein G; Protein A/G","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0004255592,0.0002771078,0.0004996051,0.0002157187,0.0003838073,0.00002370335,0.0002547338,0.0003102381,0.00001908163],"category_scores_gemma":[0.00007001192,0.0002608999,0.00006419636,0.000283883,0.0001699554,0.0001233951,0.0002375916,0.0002316241,0.00004653109],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006323103,"about_ca_system_score_gemma":0.0001618495,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000717454,"about_ca_topic_score_gemma":0.00002287273,"domain_scores_codex":[0.9983311,0.0001312907,0.0006594033,0.0004260663,0.00005156456,0.0004005579],"domain_scores_gemma":[0.9991894,0.00007664062,0.0002540115,0.0003479068,0.0001121128,0.00001990048],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001397468,0.0002102377,0.000552214,0.0001201857,0.0002213445,0.000002295921,0.000833987,0.000002411034,0.9609113,0.02615442,0.0001517951,0.01070003],"study_design_scores_gemma":[0.00126796,0.0001284731,0.00397431,0.0004197761,0.00004459311,0.00002129818,0.0007982905,0.00002295131,0.9308123,0.00161286,0.06056118,0.0003360127],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9668469,0.01227028,0.01644737,0.0002739153,0.0004248465,0.000658322,0.00002899924,0.0001177575,0.002931559],"genre_scores_gemma":[0.9910687,0.0009401765,0.005294804,0.0001831652,0.00001694308,0.00004370062,0.0002855104,0.00002153126,0.002145513],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.06040939,"threshold_uncertainty_score":0.9999843,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01279473261424524,"score_gpt":0.251205336855404,"score_spread":0.2384106042411588,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}