{"id":"W4415136222","doi":"10.1101/2025.10.11.681731","title":"High-throughput multispecies qPCR assays to study the effects of acute thermal stress in three species of <i>Acipenser</i> sturgeon","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Physiological and biochemical adaptations","field":"Environmental Science","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia; University of New Brunswick; University of Guelph; University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; University of Manitoba; Genome Canada","keywords":"Acclimatization; Transcriptome; Sturgeon; Gene expression; Gene; Real-time polymerase chain reaction; Cold stress; Messenger RNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004360944,0.0005806424,0.0003490287,0.0003765404,0.0003549193,0.0003069993,0.0003989173,0.0003952201,0.0009997946],"category_scores_gemma":[0.0002649909,0.0002990544,0.0005145856,0.0002189286,0.0003463761,0.0002578775,0.0003361198,0.0005880177,0.0003701176],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003909121,"about_ca_system_score_gemma":0.0002590335,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001655566,"about_ca_topic_score_gemma":0.006351783,"domain_scores_codex":[0.9997305,0.00003177989,0.00002204135,0.0001083344,0.00007344827,0.00003387071],"domain_scores_gemma":[0.9997486,0.00005712225,0.00006349796,0.00002717475,0.00007522017,0.00002829213],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002253655,0.000008863429,0.0008531685,0.0000231259,0.000007304617,0.000004331505,0.00001745495,0.00006256804,0.9983616,0.00001577885,0.00002502993,0.0005981695],"study_design_scores_gemma":[0.00001911801,0.000929319,0.1581965,0.0000202157,0.0001373812,0.0001825193,0.0001960514,0.004176947,0.8326586,0.000114022,0.003316243,0.00005297865],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9709779,0.0007147315,0.02455018,0.0001144842,0.00007537589,0.0001489043,0.002005701,0.0003555447,0.001057266],"genre_scores_gemma":[0.9450742,0.0006800556,0.042557,0.0005382074,0.00004082354,0.0007922185,0.005940059,0.0001353932,0.004242194],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001655566,"threshold_uncertainty_score":0.003344715,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01339283515924172,"score_gpt":0.2183387796236222,"score_spread":0.2049459444643805,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}