{"id":"W4415167573","doi":"10.1021/acsbiomedchemau.5c00152","title":"Leader-Independent C-Terminal Modification by a Radical <i>S</i> -Adenosyl- <scp>l</scp> -methionine Maturase Enables Macrocyclic GLP-1-Like Peptides","year":2025,"lang":"en","type":"article","venue":"ACS Bio & Med Chem Au","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Aegera Therapeutics (Canada)","funders":"National Institute of General Medical Sciences; National Institutes of Health","keywords":"Enzyme; Peptide; Peptide Biosynthesis; Biocatalysis; Sequence (biology); Substrate (aquarium); Cyclic peptide","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001128884,0.0002105911,0.00008993669,0.00006654731,0.00006707241,0.0002104538,0.00015435,0.0002203915,0.0009517419],"category_scores_gemma":[0.0001080521,0.00009508357,0.0001072834,0.00005493871,0.000187851,0.000203907,0.0001802155,0.0003576124,0.0003147058],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001178241,"about_ca_system_score_gemma":0.0001162186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001752252,"about_ca_topic_score_gemma":0.0002692303,"domain_scores_codex":[0.999945,0.00000743362,0.000002964955,0.00001733873,0.00001441351,0.00001276811],"domain_scores_gemma":[0.9999542,0.000008106045,0.00001721099,0.000006058896,0.000004818151,0.000009726372],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002993342,0.000004534851,0.00004582751,0.00001091092,0.000001199188,0.00002478535,0.000009582581,0.00003883944,0.9982948,0.0001368493,0.00002580306,0.001376989],"study_design_scores_gemma":[0.000002394145,0.00006132383,0.0002043931,8.52776e-7,0.000001132264,0.00004786574,0.000005177853,0.0002648723,0.9985103,0.00002254792,0.0008770966,0.000002001981],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9813399,0.0006273124,0.01445022,0.0001131605,0.0000278786,0.00002758682,0.0002053849,0.0002247989,0.002983866],"genre_scores_gemma":[0.989125,0.0003309971,0.008479371,0.00004429989,0.000007104936,0.0000195183,0.0001492123,0.00003817061,0.001806343],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009517419,"threshold_uncertainty_score":0.003183901,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008411210718724211,"score_gpt":0.2484590531512706,"score_spread":0.2400478424325464,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}