{"id":"W4415167573","doi":"10.1021/acsbiomedchemau.5c00152","title":"Leader-Independent C-Terminal Modification by a Radical <i>S</i> -Adenosyl- <scp>l</scp> -methionine Maturase Enables Macrocyclic GLP-1-Like Peptides","year":2025,"lang":"en","type":"article","venue":"ACS Bio & Med Chem Au","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Aegera Therapeutics (Canada)","funders":"National Institute of General Medical Sciences; National Institutes of Health","keywords":"Enzyme; Peptide; Peptide Biosynthesis; Biocatalysis; Sequence (biology); Substrate (aquarium); Cyclic peptide","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0002549758,0.0003486524,0.0003794114,0.00007976488,0.0001912689,0.00008803421,0.0005019503,0.0004617185,0.00002706012],"category_scores_gemma":[0.0004157388,0.0003123897,0.0002726911,0.0002859677,0.0002303536,0.00001054918,0.0002004158,0.0002542841,0.00003498232],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009519949,"about_ca_system_score_gemma":0.0001238652,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008865895,"about_ca_topic_score_gemma":0.00003840636,"domain_scores_codex":[0.9978247,0.00007277507,0.0004550905,0.000762901,0.0003275053,0.0005570794],"domain_scores_gemma":[0.9988248,0.00011423,0.0001700372,0.0005838966,0.00008856911,0.0002184731],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002592345,0.0001922372,0.0007230692,0.00006364587,0.0001810617,0.000007232421,0.00005497169,0.000003146713,0.9510772,0.0000293221,0.04439193,0.003250326],"study_design_scores_gemma":[0.0005030888,0.0000332191,0.0002551224,0.00004493704,0.000160013,0.00001524597,0.0002513566,0.00004792368,0.8691657,0.0001050838,0.1292712,0.0001471232],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9929633,0.00280833,0.000864524,0.001431488,0.0001011611,0.0001656921,0.00005838131,0.00004390803,0.001563205],"genre_scores_gemma":[0.9859818,0.0007386001,0.0003017744,0.0009076155,0.0002725543,0.00006808143,0.000715632,0.00003117502,0.01098281],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.08487929,"threshold_uncertainty_score":0.9999328,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008411210718724211,"score_gpt":0.2484590531512706,"score_spread":0.2400478424325464,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}