{"id":"W4415306500","doi":"10.1101/2025.10.15.25338083","title":"Automated Cell-free Protein Synthesis for Distributed Biomanufacturing","year":2025,"lang":"","type":"preprint","venue":"medRxiv","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Sinai Health System; Emmanuel Bible College; University of Toronto","funders":"Canadian Institutes of Health Research; University of Toronto; International Development Research Centre; Canada First Research Excellence Fund; Pfizer","keywords":"Biomanufacturing; Synthetic biology; Cell-free protein synthesis; Cloning (programming); Production (economics); Fusion protein","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003273811,0.0004672901,0.0002020955,0.0002532595,0.0002723926,0.0004180458,0.0002970559,0.0003273261,0.001330459],"category_scores_gemma":[0.0002538367,0.0001474464,0.0002294595,0.0001617915,0.000551621,0.0002782643,0.0005597064,0.0004943233,0.0006171039],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004548524,"about_ca_system_score_gemma":0.0003522448,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007503855,"about_ca_topic_score_gemma":0.001419783,"domain_scores_codex":[0.9997289,0.00004419597,0.00001529258,0.00008509886,0.00008827221,0.00003828565],"domain_scores_gemma":[0.9998245,0.00004108321,0.00005025347,0.00004114776,0.00002235309,0.00002066152],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004033628,0.00002413869,0.000247978,0.00007037175,0.000005673956,0.00004086749,0.0000321214,0.0005991001,0.9925921,0.0008319093,0.0003233944,0.005192093],"study_design_scores_gemma":[0.00002418047,0.0001726026,0.001417236,0.00001884535,0.00001092158,0.0001129511,0.0000322655,0.008783504,0.9720405,0.0005444211,0.01682021,0.00002228324],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6779364,0.002080753,0.3033402,0.0006962608,0.0003519881,0.0004265362,0.0009185434,0.002531196,0.01171813],"genre_scores_gemma":[0.8797945,0.0006236544,0.1146027,0.0001276146,0.000040163,0.0002130395,0.000461818,0.0001427255,0.003993865],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001330459,"threshold_uncertainty_score":0.004450858,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0314971155030423,"score_gpt":0.3176689780371182,"score_spread":0.2861718625340759,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}