{"id":"W4415362258","doi":"10.3390/v17101391","title":"A Highly Sensitive BRET-Based Reporter for Live-Cell Detection of HIV-1 Protease Activity and Inhibitor Screening","year":2025,"lang":"en","type":"article","venue":"Viruses","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Ministero dell'Università e della Ricerca; University of Toronto; Università degli Studi di Padova","keywords":"Proteases; Protease; Luciferase; Förster resonance energy transfer; Viability assay; In vitro; Bioluminescence; Cell culture; Transfection; Cleavage (geology)","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002039554,0.001368062,0.001434832,0.0008922784,0.0004278412,0.000954062,0.001167635,0.001873048,0.0009819421],"category_scores_gemma":[0.001140631,0.0005432102,0.0009189582,0.0009048902,0.0006886405,0.000981307,0.0008094041,0.002598062,0.000903032],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006436037,"about_ca_system_score_gemma":0.0003454181,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003149357,"about_ca_topic_score_gemma":0.0004995003,"domain_scores_codex":[0.9978465,0.0005771832,0.0001625356,0.0004779067,0.0007175708,0.0002184274],"domain_scores_gemma":[0.9994241,0.0001919317,0.0001295851,0.00008007028,0.0001276017,0.00004672668],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005353117,0.00004039566,0.00008183214,0.0001212462,0.000009696141,0.0000476469,0.00003083567,0.0002001864,0.9963433,0.0002766841,0.0001718683,0.002622617],"study_design_scores_gemma":[0.000008228519,0.00009315933,0.0001797345,0.000007511002,0.000008978714,0.0001598465,0.0000129793,0.002037975,0.9954151,0.00005861724,0.002001482,0.00001642968],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.362354,0.0105873,0.615096,0.001102558,0.0003393931,0.0006745635,0.002108694,0.002918791,0.004818582],"genre_scores_gemma":[0.5085441,0.007667648,0.469209,0.0006149722,0.00007970499,0.001366778,0.003560503,0.0003069294,0.008650398],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002039554,"threshold_uncertainty_score":0.01078629,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01643511682142856,"score_gpt":0.2814074373262646,"score_spread":0.264972320504836,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}