{"id":"W4415596066","doi":"10.1016/j.ijbiomac.2025.148568","title":"Biochemical characterization and activity enhancement of a GH13 thermostable oligo-α-1,6-glucosidase from Geobacillus stearothermophilus","year":2025,"lang":"en","type":"article","venue":"International Journal of Biological Macromolecules","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"Thompson Rivers University","funders":"Türkiye Bilimsel ve Teknolojik Araştırma Kurumu; Karadeniz Teknik Üniversitesi","keywords":"Thermostability; Thermophile; Geobacillus stearothermophilus; Enzyme; Protein engineering; Escherichia coli; Mutant; Catalytic efficiency; Hydrolysis; Docking (animal)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001310465,0.0001145696,0.0001711627,0.00007231088,0.00002504869,0.00002695142,0.0002057196,0.0001226056,0.00005165445],"category_scores_gemma":[0.0001429627,0.00009072823,0.00007871263,0.0000629178,0.0001062243,0.00001180145,0.0001206327,0.00008378433,0.000001114626],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002043295,"about_ca_system_score_gemma":0.00004340442,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008978907,"about_ca_topic_score_gemma":6.347368e-7,"domain_scores_codex":[0.9991635,0.00007891194,0.0003230645,0.0001938489,0.0001470053,0.00009365492],"domain_scores_gemma":[0.9992243,0.00001866463,0.0003098796,0.0001031642,0.0003084892,0.00003549569],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000547523,0.0001642029,0.001951521,0.000005654885,0.0001611503,0.000004124675,0.00002415437,0.000001544606,0.9836898,0.0001589609,0.00003940562,0.01325199],"study_design_scores_gemma":[0.000555777,0.0001953047,0.02519088,0.00005136456,0.00001456306,0.00001683316,0.00002251875,0.00002828283,0.9708881,0.0002536989,0.00269905,0.00008357815],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.973376,0.0003774101,0.0248627,0.000803896,0.0003225557,0.00008185446,0.0000723297,0.000004045884,0.00009917058],"genre_scores_gemma":[0.9978699,0.0009977333,0.0003946976,0.0002942648,0.0001850991,0.000004116365,0.000149571,0.000005639436,0.00009897332],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02449387,"threshold_uncertainty_score":0.369979,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009397728469341735,"score_gpt":0.2558286384694974,"score_spread":0.2464309100001557,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}