{"id":"W4415666730","doi":"10.1038/s41598-025-21406-7","title":"Real-time PCR assay design and validation for Prevotella bivia, Peptostreptococcus anaerobius, and Dialister micraerophilus, bacteria associated with increased HIV susceptibility","year":2025,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Bacterial Identification and Susceptibility Testing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research; Fogarty International Center; National Institutes of Health; George Washington University","keywords":"Peptostreptococcus; Prevotella; Bacteria; Gardnerella vaginalis; Anaerobic bacteria; Immune system; In vitro","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0031156,0.0002134036,0.0002500894,0.00009842177,0.0003712741,0.0005006147,0.00009863658,0.0001761844,0.00003241803],"category_scores_gemma":[0.001464968,0.0001959693,0.0000580981,0.0002249056,0.0003317315,0.00002521839,0.0001075314,0.000056773,0.000001439465],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004380926,"about_ca_system_score_gemma":0.0002242563,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005714622,"about_ca_topic_score_gemma":0.00008147071,"domain_scores_codex":[0.9975553,0.0003557338,0.0005175274,0.001150268,0.0001463031,0.0002749077],"domain_scores_gemma":[0.9982579,0.0001218564,0.0003362905,0.0007547045,0.0004102217,0.000119042],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000954812,0.00008624138,0.03478356,0.0000480667,0.00004453925,0.000002987242,0.00005231984,0.000005637548,0.9608792,0.000006636466,0.003620176,0.0003751692],"study_design_scores_gemma":[0.001264681,0.0002782622,0.1618105,0.0001759373,0.0001840631,0.00003408273,0.0000614951,0.0005784355,0.8288432,0.0009061906,0.005268808,0.0005942889],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955087,0.00003351319,0.002269915,0.0001244997,0.0005126669,0.00135017,0.00003211755,0.00004758799,0.0001207967],"genre_scores_gemma":[0.9897768,0.000009083788,0.003959166,0.00002154215,0.00004857279,0.00008390129,0.001703568,0.0000207493,0.004376648],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.132036,"threshold_uncertainty_score":0.7991395,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01310922506731997,"score_gpt":0.2463469445251301,"score_spread":0.2332377194578102,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}