{"id":"W4415715237","doi":"10.1016/j.xpro.2025.104173","title":"Protocol for halofuginone-mediated metabolic reprogramming of murine T cells via activation of the GCN2 pathway","year":2025,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Immune Cell Function and Interaction","field":"Immunology and Microbiology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Princess Margaret Cancer Centre","funders":"Canadian Institutes of Health Research","keywords":"T cell; Reprogramming; Cytotoxic T cell; Transgene; Cell; Integrated stress response; Adoptive cell transfer; Fight-or-flight response; T-cell receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004192558,0.0009051322,0.0004099882,0.0006920178,0.0006795847,0.0002707942,0.0007467075,0.000626252,0.01253893],"category_scores_gemma":[0.00023518,0.0003923153,0.0005386673,0.0004147825,0.0002937577,0.0002948913,0.0004018339,0.001638713,0.006771454],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003521533,"about_ca_system_score_gemma":0.000529427,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005662142,"about_ca_topic_score_gemma":0.001802453,"domain_scores_codex":[0.999535,0.00008464774,0.00005435403,0.0001123869,0.0001372795,0.00007643772],"domain_scores_gemma":[0.9997954,0.00003631878,0.00002684763,0.00005478332,0.00004339842,0.00004335527],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0005217197,0.0001830435,0.0001863405,0.000369929,0.00001460615,0.000218125,0.0001101018,0.0004831026,0.9807338,0.00158107,0.004943889,0.01065429],"study_design_scores_gemma":[0.0001980538,0.001148689,0.001655093,0.0001190896,0.00005957508,0.0005737023,0.00004556762,0.001637752,0.7793715,0.0005010859,0.2146419,0.00004800134],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.3109594,0.01712072,0.5330899,0.001751125,0.002924832,0.0209426,0.02849131,0.009801679,0.07491845],"genre_scores_gemma":[0.3994676,0.01394557,0.403415,0.001822163,0.0003742987,0.04492755,0.03954871,0.001335376,0.0951637],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.01253893,"threshold_uncertainty_score":0.04194695,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02232768766942958,"score_gpt":0.3107062927689925,"score_spread":0.2883786050995629,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}