{"id":"W4415771629","doi":"10.1101/2025.10.31.685832","title":"Co-opting the bacterial lipoprotein pathway for the biosynthesis of lipidated macrocyclic peptides","year":2025,"lang":"en","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health","keywords":"Diacylglycerol kinase; Biosynthesis; Subfamily; Enzyme; Peptide; Peptide sequence; Amino acid; Myxococcus xanthus","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0008233998,0.0003800979,0.0003902289,0.00008173321,0.0002678421,0.0001268434,0.0008020953,0.0004620032,0.00001661384],"category_scores_gemma":[0.0007987187,0.0002644242,0.000314813,0.00022892,0.0001806994,0.0000068267,0.0003473442,0.0002308121,0.000003175199],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000375596,"about_ca_system_score_gemma":0.0003926632,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005069687,"about_ca_topic_score_gemma":0.00001071325,"domain_scores_codex":[0.9981049,0.0001561095,0.0005557191,0.0006753847,0.0001822011,0.0003256592],"domain_scores_gemma":[0.9973573,0.000142799,0.0005480111,0.001442108,0.0004525035,0.00005729171],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008115732,0.00005384546,0.0002527915,0.0002377777,0.0003208079,2.838661e-7,0.00000663188,0.00008924624,0.9982744,0.00008965052,0.0005602386,0.00003315793],"study_design_scores_gemma":[0.0002661854,0.00005392179,0.002806188,0.0001686739,0.0001726584,6.155516e-9,0.000009128206,0.000197961,0.9676124,6.417145e-7,0.028424,0.0002882705],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9895381,0.002000537,0.004508576,0.0002664986,0.0007508457,0.001599373,0.001271485,0.00004978251,0.00001479296],"genre_scores_gemma":[0.996984,0.0005126799,0.0009659239,0.0001217808,0.0007984068,0.0005136137,0.00001350082,0.00005782881,0.00003223769],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03066204,"threshold_uncertainty_score":0.9999808,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01130744656730934,"score_gpt":0.2299081235185628,"score_spread":0.2186006769512535,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}