{"id":"W4415895252","doi":"10.1021/jacs.5c06736","title":"Engineering Polyketide Stereocenters with Ketoreductase Domain Exchanges","year":2025,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Biological and Environmental Research; Office of Science; Lawrence Berkeley National Laboratory; Novo Nordisk; U.S. Department of Energy; Philomathia Foundation; Novo Nordisk Fonden; Svenska Forskningsrådet Formas","keywords":"Polyketide; Stereocenter; Domain (mathematical analysis); Epothilones; Polyketide synthase; Directed evolution","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005369907,0.0006250077,0.0003459844,0.0002623514,0.0001683075,0.0005590551,0.0003972163,0.0004041517,0.0007150178],"category_scores_gemma":[0.0006223017,0.0002353563,0.0003930171,0.0002824856,0.0002953657,0.0005633727,0.0005964573,0.0009188955,0.000384262],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003596589,"about_ca_system_score_gemma":0.0003603301,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002505384,"about_ca_topic_score_gemma":0.0004893841,"domain_scores_codex":[0.9995762,0.00007228543,0.00005030452,0.00009845633,0.0001185435,0.00008423963],"domain_scores_gemma":[0.999686,0.00005910247,0.0001259443,0.0000455309,0.00003922809,0.00004426469],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008859731,0.0001090335,0.0005411774,0.00006882523,0.00001458596,0.0001412598,0.00005696015,0.001439225,0.9904845,0.0006706396,0.00004175492,0.006343568],"study_design_scores_gemma":[0.00001348841,0.000296967,0.0004043122,0.000006370527,0.00001642369,0.0001166663,0.00002391803,0.002033327,0.994794,0.0001031072,0.002181798,0.00000964698],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9791526,0.0004684507,0.0179865,0.00007573681,0.00003413494,0.00008835417,0.00009544912,0.0001540443,0.001944699],"genre_scores_gemma":[0.9793907,0.0005354458,0.01852794,0.00005653636,0.000006581851,0.00004619035,0.0001806016,0.00006776784,0.0011883],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007150178,"threshold_uncertainty_score":0.002839923,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005009898391682843,"score_gpt":0.2234382337991473,"score_spread":0.2184283354074645,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}