{"id":"W4415962474","doi":"10.1128/spectrum.01732-25","title":"Assessment of protocols for characterization of the human skin microbiome using shotgun metagenomics and comparative analysis with 16S metabarcoding","year":2025,"lang":"en","type":"article","venue":"Microbiology Spectrum","topic":"Dermatology and Skin Diseases","field":"Medicine","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Intertek (Canada)","funders":"Agence Nationale de la Recherche","keywords":"Microbiome; Metagenomics; Shotgun sequencing; Shotgun; Human Microbiome Project; Human microbiome; 16S ribosomal RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.008047971,0.001590852,0.001204351,0.002669081,0.001066236,0.001368398,0.00129678,0.001071128,0.001291976],"category_scores_gemma":[0.009541542,0.0008795153,0.001656876,0.002003294,0.0008603811,0.0007846372,0.00174399,0.001288076,0.001545915],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005112604,"about_ca_system_score_gemma":0.001722843,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001554834,"about_ca_topic_score_gemma":0.003253476,"domain_scores_codex":[0.9929799,0.002459514,0.0009602749,0.001115635,0.002107274,0.0003774564],"domain_scores_gemma":[0.9961206,0.001062692,0.0005446665,0.000565534,0.001567154,0.0001393289],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002227794,0.000150304,0.004770373,0.0007995128,0.0001447324,0.0001507023,0.0004340484,0.0007742082,0.9698037,0.0003555421,0.0003546529,0.02203947],"study_design_scores_gemma":[0.00002342565,0.000888727,0.01488686,0.0002545306,0.0003001667,0.0003968365,0.0005238204,0.006926114,0.9662644,0.0005476139,0.00888769,0.00009980459],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3600174,0.004596102,0.6201618,0.000570016,0.0003843519,0.004942795,0.004812612,0.001731103,0.002783684],"genre_scores_gemma":[0.1557406,0.002205315,0.8307778,0.0003123855,0.00005564327,0.003897893,0.005535983,0.0004851092,0.0009892358],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.008047971,"threshold_uncertainty_score":0.04256225,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03486569399953687,"score_gpt":0.3537489314140832,"score_spread":0.3188832374145463,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}