{"id":"W4416045049","doi":"10.1016/j.xpro.2025.104188","title":"Protocol for cell-specific RNA expression profiling and lipidomics analyses of Drosophila melanogaster intestinal progenitor cells","year":2025,"lang":"en","type":"article","venue":"STAR Protocols","topic":"Invertebrate Immune Response Mechanisms","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"National Center for Research Resources; Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; National Institutes of Health","keywords":"Transcriptome; Drosophila melanogaster; Lipidomics; RNA; Progenitor cell; Lipidome; Gene expression profiling; Schneider 2 cells; Cell sorting","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001085729,0.002111268,0.001326778,0.002752914,0.002332023,0.0007291172,0.001874169,0.000859796,0.0275263],"category_scores_gemma":[0.0007048417,0.001844173,0.001179235,0.001741924,0.0006890174,0.0005388657,0.001049287,0.003405846,0.01959121],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009703628,"about_ca_system_score_gemma":0.001658267,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001803475,"about_ca_topic_score_gemma":0.008694629,"domain_scores_codex":[0.9985232,0.0002362063,0.0002255201,0.000363224,0.000480261,0.0001715204],"domain_scores_gemma":[0.9992551,0.0001431189,0.00005876934,0.0002505932,0.000191184,0.0001012591],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0003030819,0.0001675833,0.0003410924,0.0007151036,0.00005114923,0.0003610825,0.0001412783,0.0005277459,0.9710391,0.001889031,0.01249136,0.01197246],"study_design_scores_gemma":[0.0003012109,0.000802435,0.007516933,0.0003335783,0.0001583919,0.001110292,0.00009474676,0.003658777,0.5923575,0.001740767,0.3917323,0.0001930047],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.05505517,0.005327204,0.7712033,0.001010113,0.001253169,0.03279728,0.0862721,0.01071487,0.0363668],"genre_scores_gemma":[0.05064862,0.005510693,0.6754187,0.001176132,0.0002145344,0.07419392,0.1412942,0.002286709,0.04925653],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.0275263,"threshold_uncertainty_score":0.09208459,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05863806753737148,"score_gpt":0.3605117162850222,"score_spread":0.3018736487476507,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}