{"id":"W4416300078","doi":"10.1101/2025.11.13.688287","title":"Conjugation-mediated DNA delivery to the filamentous fungus <i>Ustilago maydis</i>","year":2025,"lang":"","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Extrachromosomal DNA; Horizontal gene transfer; DNA; Plasmid; Gene; Electroporation; Filamentous fungus; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000220665,0.0003746681,0.0001234282,0.0002309694,0.0001756941,0.0002720272,0.0002456954,0.0003120506,0.001194433],"category_scores_gemma":[0.0001689304,0.0001364193,0.0002481139,0.0001818434,0.0002830264,0.0001378965,0.0002727403,0.000444776,0.0005618966],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003308781,"about_ca_system_score_gemma":0.0002121075,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001144973,"about_ca_topic_score_gemma":0.001039177,"domain_scores_codex":[0.9998319,0.00003998779,0.00001636763,0.00004287569,0.00003791084,0.00003094015],"domain_scores_gemma":[0.9998832,0.00002484886,0.00004440239,0.00002219564,0.00001081561,0.00001450801],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001427661,0.000009190845,0.00004461923,0.00001909911,0.00000199527,0.00003151621,0.00001412487,0.00003342317,0.9989047,0.0001047186,0.0000402788,0.0007820057],"study_design_scores_gemma":[0.0000027673,0.00006106208,0.0005011584,0.000001950607,0.000004881039,0.00006682914,0.000005912093,0.0001675572,0.9973977,0.00001898093,0.001769258,0.00000208231],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9485396,0.0007856888,0.04385372,0.0003626878,0.0001036491,0.0002451281,0.0008509186,0.0005414879,0.004717013],"genre_scores_gemma":[0.9665841,0.0006834626,0.02054524,0.00008537235,0.000014926,0.00007883177,0.001213986,0.0001188951,0.01067507],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001194433,"threshold_uncertainty_score":0.003995836,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007616411233701245,"score_gpt":0.2073824904716848,"score_spread":0.1997660792379836,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}