{"id":"W4416341307","doi":"10.1101/2025.11.17.688837","title":"A universal buffer system for native LC-MS analysis of antibody-based therapeutics","year":2025,"lang":"","type":"preprint","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Analyte; Elution; Ammonium acetate; Mass spectrometry; Acetic acid; Hydrophilic interaction chromatography; Ammonium","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002262387,0.001918559,0.0009289281,0.001571861,0.0007182426,0.001044623,0.001513015,0.001170048,0.005784246],"category_scores_gemma":[0.001392391,0.0005804094,0.0006346104,0.0006689634,0.0006534963,0.001138277,0.001404109,0.001253208,0.00292048],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008038836,"about_ca_system_score_gemma":0.001698615,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004148815,"about_ca_topic_score_gemma":0.0006305823,"domain_scores_codex":[0.9982665,0.0002985678,0.0002102284,0.0005472535,0.0004893055,0.0001880703],"domain_scores_gemma":[0.9994904,0.0001263602,0.0000753553,0.00008351276,0.0001306707,0.00009371895],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000297622,0.00007778726,0.0005609088,0.0002684499,0.00004970025,0.00009330892,0.00005306649,0.0002158988,0.97293,0.001160152,0.002001011,0.02229216],"study_design_scores_gemma":[0.0000872013,0.0003425977,0.001371891,0.00005855189,0.0000463721,0.0006688791,0.00002744586,0.005631565,0.9586073,0.0006502022,0.03243799,0.00006991609],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1652153,0.008927514,0.7839823,0.0007385456,0.001045049,0.002277523,0.005655047,0.02636839,0.005790356],"genre_scores_gemma":[0.2809109,0.004227223,0.6804674,0.002203029,0.0002087839,0.007711216,0.01227464,0.002349929,0.009646942],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.005784246,"threshold_uncertainty_score":0.01935023,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01409015706668765,"score_gpt":0.2590514664974642,"score_spread":0.2449613094307766,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}