{"id":"W4416594753","doi":"10.1186/s12870-025-07686-1","title":"A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L","year":2025,"lang":"en","type":"article","venue":"BMC Plant Biology","topic":"Cannabis and Cannabinoid Research","field":"Medicine","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Protoplast; Cannabis sativa; Genome; Protocol (science); Gene; Genome editing","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005677544,0.0007923205,0.0006242784,0.0009879341,0.001223388,0.000493362,0.0009713349,0.0006420368,0.002541074],"category_scores_gemma":[0.0003750973,0.0005073429,0.0006584825,0.0006149512,0.0004712658,0.0004625516,0.0009645793,0.001654819,0.002766652],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007107587,"about_ca_system_score_gemma":0.0009111957,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002307705,"about_ca_topic_score_gemma":0.005294735,"domain_scores_codex":[0.9992748,0.00009635202,0.0001059357,0.000164063,0.0002844883,0.00007440538],"domain_scores_gemma":[0.9997494,0.00004806209,0.00004331485,0.00006883762,0.00005520202,0.00003524865],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002161326,0.00001692995,0.00002982111,0.00009509434,0.000003819509,0.0001153075,0.0000567096,0.0000453336,0.996547,0.0002222061,0.0002090361,0.00263713],"study_design_scores_gemma":[0.00002127617,0.0001609808,0.001169609,0.00004373463,0.00003986153,0.0008650692,0.00003853859,0.001012767,0.9444224,0.0002117233,0.05195915,0.0000549166],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.260705,0.00981232,0.6794577,0.001170333,0.00112934,0.007128706,0.008853541,0.007928289,0.02381488],"genre_scores_gemma":[0.3734466,0.008189949,0.5510053,0.0005063858,0.0002091983,0.006954732,0.02019232,0.00149918,0.03799639],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002541074,"threshold_uncertainty_score":0.008500695,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02710743318191831,"score_gpt":0.3508067799700695,"score_spread":0.3236993467881512,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}