{"id":"W4416601936","doi":"10.5256/f1000research.160203.r330318","title":"Referee report. For: A guide to selecting high-performing antibodies for PLC-gamma-2 for use in Western Blot, immunoprecipitation and immunofluorescence [version 1; peer review: 2 approved]","year":2024,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Canadian Institutes of Health Research; School of Medicine, Indiana University; Genome Canada; Ontario Genomics; Mitacs; Government of Canada; Purdue University","keywords":"Immunoprecipitation; Immunofluorescence; Antibody; Indirect immunofluorescence; Specific antibody","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["metaresearch","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.007808926,0.001077836,0.001397244,0.004725536,0.00331954,0.003596105,0.003058856,0.004473229,0.6052611],"category_scores_gemma":[0.06641468,0.0008751745,0.001345164,0.003401675,0.000818942,0.00265849,0.003407703,0.003945219,0.4729806],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002776842,"about_ca_system_score_gemma":0.007026229,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01720025,"about_ca_topic_score_gemma":0.0362209,"domain_scores_codex":[0.9944509,0.0008527889,0.0006561203,0.000599252,0.00307312,0.0003677957],"domain_scores_gemma":[0.9241394,0.008848989,0.001386059,0.00433444,0.05850035,0.002790692],"domain_codex":null,"domain_gemma":"evaluation","domain_candidate":"evaluation","domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000006565438,0.00001144668,0.00003281996,0.00006021685,0.000001509817,0.00002598351,0.00001590347,0.000008148859,0.0001864982,0.0001958412,0.9918506,0.007604324],"study_design_scores_gemma":[0.00001298481,0.00001301526,0.0005228545,0.0001142415,0.000006584376,0.00009726312,0.00009561685,0.00003996333,0.0003633977,0.0007444533,0.997965,0.000024595],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"editorial","genre_gemma":"commentary","genre_scores_codex":[0.001198097,0.006473573,0.02710318,0.2300896,0.3593423,0.005313817,0.08516955,0.01495875,0.2703511],"genre_scores_gemma":[0.003169833,0.003532618,0.01470682,0.03396628,0.0217804,0.00244647,0.02087062,0.003719203,0.8958077],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.9921911,"threshold_uncertainty_score":0.5630471,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0773840882737422,"score_gpt":0.4314631489629935,"score_spread":0.3540790606892513,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}