{"id":"W4416602366","doi":"10.1021/acssynbio.5c00689","title":"Tri-Functional CRISPR Screen Reveals Overexpression of <i>QDR2</i> and <i>QDR3</i> Transporters Increase Fumaric Acid Production in <i>Kluyveromyces marxianus</i>","year":2025,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Genome Canada","keywords":"Fumaric acid; Metabolic engineering; CRISPR; Succinic acid; Lactose; Function (biology); Gene; Mitochondrion; Biosynthesis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004606932,0.0002078392,0.0003014941,0.0001876692,0.00007419727,0.00001012357,0.0002106861,0.0003065229,0.00002381996],"category_scores_gemma":[0.0001681235,0.0001823352,0.00007451489,0.0002578101,0.0003493052,0.000007768749,0.0001587831,0.0001842055,0.000002622124],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000165955,"about_ca_system_score_gemma":0.0001111498,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001796331,"about_ca_topic_score_gemma":0.00005267889,"domain_scores_codex":[0.9982839,0.0002366602,0.0004071636,0.0006136342,0.0001267291,0.0003318742],"domain_scores_gemma":[0.999272,0.00005341509,0.0001020163,0.0003870459,0.0001053661,0.00008011466],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006833773,0.000133398,0.0299767,0.00009300057,0.00004216011,0.000001404748,0.00001617245,0.00001307211,0.9603766,0.0001740254,0.001535186,0.006954967],"study_design_scores_gemma":[0.0009555891,0.0002635811,0.02304053,0.0001530801,0.0000310378,0.00002728864,0.00005055829,0.00001392873,0.9703847,0.00104842,0.003822707,0.0002086215],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.994538,0.002252316,0.0003393936,0.0007048069,0.0001761601,0.0003376169,0.0001042275,0.00001012347,0.00153735],"genre_scores_gemma":[0.9973525,0.00108034,0.0002744692,0.0002490071,0.00008179085,0.00004719545,0.0001873765,0.00001487308,0.0007124289],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01000811,"threshold_uncertainty_score":0.7435412,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01022762583663332,"score_gpt":0.2553329907960509,"score_spread":0.2451053649594175,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}