{"id":"W4416602366","doi":"10.1021/acssynbio.5c00689","title":"Tri-Functional CRISPR Screen Reveals Overexpression of <i>QDR2</i> and <i>QDR3</i> Transporters Increase Fumaric Acid Production in <i>Kluyveromyces marxianus</i>","year":2025,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Genome Canada","keywords":"Fumaric acid; Metabolic engineering; CRISPR; Succinic acid; Lactose; Function (biology); Gene; Mitochondrion; Biosynthesis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001857597,0.000557061,0.0004121021,0.0003245414,0.0001908144,0.0004183994,0.000382501,0.0004187091,0.001873078],"category_scores_gemma":[0.0001609727,0.0002166091,0.0004965371,0.0002485884,0.0002432922,0.0001598895,0.0004517111,0.0005664919,0.0008283483],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004156104,"about_ca_system_score_gemma":0.0002689637,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002386617,"about_ca_topic_score_gemma":0.00296524,"domain_scores_codex":[0.9997122,0.00002317917,0.00004682902,0.00007134604,0.00008669576,0.00005979946],"domain_scores_gemma":[0.9998468,0.00002512225,0.00004941242,0.00001896635,0.00001372864,0.00004591014],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006897295,0.00001676981,0.0002354455,0.00003575124,0.000007847816,0.00008745821,0.00001598038,0.0001083342,0.998413,0.00007776559,0.00007425761,0.0008584224],"study_design_scores_gemma":[0.00002450937,0.0001484863,0.007408518,0.00001171239,0.00005412997,0.0003426308,0.0000734701,0.002142822,0.9857402,0.00006458184,0.003968835,0.00001996626],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9833565,0.0005507257,0.007683352,0.0002117929,0.00004908229,0.00006120594,0.003324393,0.0009715399,0.003791241],"genre_scores_gemma":[0.9883543,0.0003447925,0.004009886,0.0001284675,0.000004976612,0.00004232408,0.002458855,0.0001434132,0.004513051],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002386617,"threshold_uncertainty_score":0.006266057,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01022762583663332,"score_gpt":0.2553329907960509,"score_spread":0.2451053649594175,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}