{"id":"W4416607628","doi":"10.5256/f1000research.146199.r178227","title":"Referee report. For: The identification of high-performing antibodies for RNA-binding protein FUS for use in Western Blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved, 1 approved with reservations]","year":2023,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Systemic Lupus Erythematosus Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Genentech; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Medical Research Council; Bayer; Motor Neurone Disease Association; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Identification (biology); Indirect immunofluorescence; Monoclonal antibody; Western blot","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.006332278,0.0008811871,0.001493082,0.003169762,0.003601951,0.002889106,0.002700734,0.006204478,0.5299819],"category_scores_gemma":[0.07689013,0.0006080852,0.001270067,0.002505907,0.000811313,0.002231875,0.002556526,0.004373138,0.3387719],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.003710833,"about_ca_system_score_gemma":0.005366352,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.02982377,"about_ca_topic_score_gemma":0.04452379,"domain_scores_codex":[0.9952572,0.0006524749,0.0005925702,0.0005677207,0.002577212,0.0003528203],"domain_scores_gemma":[0.9129357,0.01230196,0.001235888,0.003997419,0.06671765,0.002811494],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00000956855,0.00001185599,0.00003508411,0.00003819915,0.000001931955,0.0000346263,0.00001327137,0.000006350759,0.0001342756,0.0002145039,0.9963892,0.003111279],"study_design_scores_gemma":[0.00002479257,0.00001584644,0.001005134,0.00008080748,0.000009660902,0.0001337646,0.000104634,0.00007641408,0.0003915913,0.001029078,0.9970945,0.00003387202],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"editorial","genre_gemma":"commentary","genre_scores_codex":[0.0009787575,0.003046506,0.007738834,0.3298502,0.4317456,0.002458288,0.04917265,0.006981858,0.1680273],"genre_scores_gemma":[0.006143709,0.002540983,0.006681913,0.08279446,0.04349538,0.001426283,0.01667063,0.002436947,0.8378097],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.5299819,"threshold_uncertainty_score":0.6704237,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09656299780518428,"score_gpt":0.3924384293496122,"score_spread":0.2958754315444279,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}