{"id":"W4416610070","doi":"10.5256/f1000research.155278.r202703","title":"Referee report. For: Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 1 approved]","year":2023,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Metal-Catalyzed Oxygenation Mechanisms","field":"Chemistry","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Genentech; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Bayer; Motor Neurone Disease Association; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Superoxide dismutase; Antibody; Identification (biology); Indirect immunofluorescence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch"],"consensus_categories":[],"category_scores_codex":[0.007540095,0.0002124344,0.000515584,0.000398511,0.0002450837,0.00007746973,0.0004793507,0.000170086,0.0000302902],"category_scores_gemma":[0.01224698,0.0002031388,0.0001596694,0.0004750486,0.0001813826,0.0005983022,0.0002203282,0.0002148617,0.000009662495],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001423999,"about_ca_system_score_gemma":0.0001407063,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005010604,"about_ca_topic_score_gemma":0.0001679786,"domain_scores_codex":[0.9961826,0.00009517396,0.001267178,0.0006229457,0.001342636,0.0004894821],"domain_scores_gemma":[0.9923342,0.001225138,0.0004962489,0.0007973238,0.005063909,0.00008317092],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003748922,0.0001015216,0.0011971,0.006372486,0.0000754776,0.000002904189,0.0006933173,0.00001710093,0.9692047,0.0003519725,0.01898172,0.002626822],"study_design_scores_gemma":[0.001815062,0.0001164676,0.003910707,0.001565806,0.0000637865,0.000008470194,0.001356516,0.001648189,0.9601103,0.001118664,0.02802018,0.0002658641],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.984303,0.0007965926,0.004154038,0.003489882,0.00009511999,0.001995546,0.00509007,0.00005750655,0.00001825826],"genre_scores_gemma":[0.8296025,0.004691027,0.02039366,0.00002996817,0.0001213458,0.002492774,0.05736802,0.0001641764,0.08513651],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1547005,"threshold_uncertainty_score":0.9960733,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07546286737185531,"score_gpt":0.3778775584135601,"score_spread":0.3024146910417048,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}