{"id":"W4416631079","doi":"10.5256/f1000research.175953.r352668","title":"Referee report. For: A guide to selecting high-performing antibodies for ADNP (UniProt ID: Q9H2P0) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 2 approved, 1 approved with reservations]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Genentech; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Simons Foundation Autism Research Initiative; Bayer; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Virus","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.009505006,0.001247219,0.001713046,0.006581567,0.003519736,0.005507479,0.003847311,0.0039604,0.7436457],"category_scores_gemma":[0.09115557,0.001032228,0.001340454,0.005098111,0.001041184,0.003952299,0.005008856,0.003389202,0.6983013],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002358726,"about_ca_system_score_gemma":0.007525556,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01438377,"about_ca_topic_score_gemma":0.02960739,"domain_scores_codex":[0.9926978,0.001257016,0.001024017,0.0007988102,0.003764172,0.000458259],"domain_scores_gemma":[0.8951426,0.01071054,0.001807616,0.007528502,0.0804156,0.004395128],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000006856875,0.000007725474,0.00002325448,0.00008429529,0.000001498466,0.00001715374,0.00001508814,0.000005495931,0.0001345218,0.0001397513,0.9927548,0.00680975],"study_design_scores_gemma":[0.00001260932,0.000007913557,0.0002978926,0.0001227387,0.000004853385,0.00006765807,0.00006538549,0.00002334916,0.0001935201,0.000578533,0.9986085,0.00001714893],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"other","genre_gemma":"commentary","genre_scores_codex":[0.001078791,0.00648197,0.02580241,0.1500359,0.2912545,0.004923817,0.1856398,0.02567073,0.3091121],"genre_scores_gemma":[0.002542442,0.00395334,0.01544782,0.0210998,0.02041491,0.002321269,0.05985667,0.009470402,0.8648934],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.7436457,"threshold_uncertainty_score":0.3656582,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09805627413112457,"score_gpt":0.4262034185809321,"score_spread":0.3281471444498076,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}