{"id":"W4416631373","doi":"10.5256/f1000research.186850.r416175","title":"Referee report. For: A guide to selecting high-performing antibodies for VCP (UniProt ID: P55072) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Genentech; Mitacs; Government of Canada; Québec Consortium for Drug Discovery; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Ontario Genomics; Genome Canada; Bayer; Motor Neurone Disease Association; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Virus","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.009971507,0.001283277,0.001690133,0.006473777,0.003359531,0.005235835,0.003631795,0.003310592,0.7414558],"category_scores_gemma":[0.0924739,0.0009949361,0.001277819,0.00518534,0.001021272,0.003915616,0.005517717,0.003121771,0.6540407],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002320414,"about_ca_system_score_gemma":0.007721113,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01210278,"about_ca_topic_score_gemma":0.0259175,"domain_scores_codex":[0.9924397,0.001372115,0.001042889,0.0008561657,0.003810744,0.0004783394],"domain_scores_gemma":[0.8926092,0.01120838,0.001887572,0.008615808,0.0811621,0.004517065],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000008514727,0.000007669319,0.00002921022,0.0001134915,0.000002003017,0.00001935157,0.00002023996,0.000006708444,0.0001806254,0.0001688029,0.9915051,0.007938336],"study_design_scores_gemma":[0.00001263909,0.000008477899,0.0003246279,0.0001429938,0.000006144554,0.0000741634,0.00007276738,0.00002439723,0.0002651978,0.000651735,0.9983985,0.00001838539],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"other","genre_gemma":"commentary","genre_scores_codex":[0.001338264,0.007238934,0.03134793,0.1391075,0.273001,0.004639797,0.2429555,0.02704287,0.2733282],"genre_scores_gemma":[0.003914988,0.005143849,0.0211606,0.01941166,0.02167398,0.002797052,0.08757356,0.01195917,0.8263652],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.7414558,"threshold_uncertainty_score":0.3687819,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0961891693431498,"score_gpt":0.4410388689041147,"score_spread":0.3448496995609649,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}