{"id":"W4416631373","doi":"10.5256/f1000research.186850.r416175","title":"Referee report. For: A guide to selecting high-performing antibodies for VCP (UniProt ID: P55072) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Genentech; Mitacs; Government of Canada; Québec Consortium for Drug Discovery; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Ontario Genomics; Genome Canada; Bayer; Motor Neurone Disease Association; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Virus","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch","metaepi_narrow"],"consensus_categories":["metaresearch"],"category_scores_codex":[0.03849184,0.001147014,0.003258992,0.002191127,0.001035294,0.000397098,0.002067114,0.0007138224,0.00004132492],"category_scores_gemma":[0.09944795,0.001098753,0.0006288577,0.00237052,0.0003838422,0.001025083,0.001484547,0.001706543,0.00005882107],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001721248,"about_ca_system_score_gemma":0.002262325,"about_ca_topic_candidate":true,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.007869893,"about_ca_topic_score_gemma":0.005631162,"domain_scores_codex":[0.9862151,0.0008932301,0.003612555,0.002544606,0.004483256,0.002251298],"domain_scores_gemma":[0.9512458,0.004741827,0.001747138,0.002337357,0.03959275,0.0003351304],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0008894055,0.0001369412,0.0002546862,0.08904174,0.0003242048,0.00001154172,0.0003604155,0.00000643634,0.00474426,0.00004301373,0.8936951,0.01049228],"study_design_scores_gemma":[0.001992734,0.0007843827,0.0003374286,0.1161358,0.0003783149,0.00004249878,0.000260107,0.0001045759,0.002213658,0.000125036,0.8767259,0.0008995738],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"commentary","genre_gemma":"other","genre_scores_codex":[0.007082375,0.3429627,0.007681211,0.3644168,0.002432234,0.1506859,0.1224884,0.0007468745,0.001503498],"genre_scores_gemma":[0.00008675774,0.04005631,0.08408363,0.0005869305,0.0003494052,0.01315897,0.07132108,0.0005042929,0.7898526],"genre_candidate":"other","genre_consensus":null,"teacher_disagreement_score":0.7883492,"threshold_uncertainty_score":0.9991463,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0961891693431498,"score_gpt":0.4410388689041147,"score_spread":0.3448496995609649,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}