{"id":"W4416639802","doi":"10.5256/f1000research.145562.r247622","title":"Referee report. For: The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence [version 1; peer review: 1 approved, 1 approved with reservations]","year":2024,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Genentech; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Bayer; Motor Neurone Disease Association; Pfizer; Bristol-Myers Squibb","keywords":"Immunoprecipitation; Immunofluorescence; Identification (biology); Antibody; Monoclonal antibody; Indirect immunofluorescence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["metaresearch","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.005911949,0.000876086,0.001456761,0.002794274,0.003586635,0.002993156,0.00272678,0.007364964,0.4759861],"category_scores_gemma":[0.0744381,0.0005810252,0.00123687,0.002243625,0.0008373648,0.002185777,0.002370758,0.004608443,0.3142294],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.003534044,"about_ca_system_score_gemma":0.005327003,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.02695419,"about_ca_topic_score_gemma":0.04079369,"domain_scores_codex":[0.9953654,0.0005892247,0.0005505714,0.0005471406,0.002602133,0.0003455638],"domain_scores_gemma":[0.9234518,0.0117972,0.001156696,0.003474141,0.05771009,0.00240999],"domain_codex":null,"domain_gemma":"evaluation","domain_candidate":"evaluation","domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000009397732,0.00001118192,0.00003688495,0.00003620404,0.000001839482,0.00004149457,0.00001293419,0.000006439065,0.0001329188,0.0002417045,0.9962246,0.003244245],"study_design_scores_gemma":[0.00002198475,0.000016587,0.0009771383,0.00008477295,0.000009574987,0.0001587054,0.0001095415,0.00007869634,0.0003745068,0.001054069,0.9970805,0.00003384106],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"editorial","genre_gemma":"commentary","genre_scores_codex":[0.001000059,0.003415544,0.006598007,0.3803935,0.4076231,0.002202865,0.02974212,0.006360336,0.1626644],"genre_scores_gemma":[0.005856413,0.002317467,0.005764167,0.09075792,0.04220363,0.001184532,0.009456209,0.001811431,0.8406482],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.9940881,"threshold_uncertainty_score":0.7474422,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09479813789126448,"score_gpt":0.4064691981680716,"score_spread":0.3116710602768071,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}