{"id":"W4416647920","doi":"10.5256/f1000research.176211.r360584","title":"Referee report. For: A guide to selecting high-performing antibodies for Huntingtin (UniProt ID: P42858) for use in western blot, immunoprecipitation, and immunofluorescence [version 2; peer review: 3 approved]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Mitacs; Government of Canada; Ontario Genomics; Genome Canada","keywords":"Immunofluorescence; Antibody; Huntingtin; Indirect immunofluorescence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch","metaepi_narrow"],"consensus_categories":["metaresearch"],"category_scores_codex":[0.04359079,0.001156983,0.003255121,0.002236625,0.001127182,0.0004436062,0.002062765,0.0007138104,0.00003602104],"category_scores_gemma":[0.1227164,0.001132936,0.0006301475,0.002473184,0.00037318,0.0009874656,0.001599561,0.001788899,0.00005483763],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001472311,"about_ca_system_score_gemma":0.002058029,"about_ca_topic_candidate":true,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00873253,"about_ca_topic_score_gemma":0.005596686,"domain_scores_codex":[0.98589,0.0008503896,0.003861912,0.002621181,0.004403003,0.002373477],"domain_scores_gemma":[0.9499837,0.005115288,0.002091086,0.002266771,0.04023097,0.0003122371],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0007597358,0.0001551662,0.0005143086,0.09274179,0.0002872133,0.000009110536,0.000383361,0.000006071802,0.006295274,0.00003363699,0.8870737,0.01174064],"study_design_scores_gemma":[0.001980439,0.0006599606,0.0004604959,0.1236089,0.0003582848,0.00003423302,0.0003086048,0.0001461515,0.00298742,0.00007196877,0.8684714,0.0009121546],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"commentary","genre_gemma":"other","genre_scores_codex":[0.01554318,0.2800559,0.007824658,0.4058158,0.002748745,0.1656278,0.1194649,0.0009480499,0.001970986],"genre_scores_gemma":[0.0001230511,0.02545838,0.1046569,0.0006062242,0.0003909826,0.01207152,0.06779487,0.0005528936,0.7883452],"genre_candidate":"other","genre_consensus":null,"teacher_disagreement_score":0.7863742,"threshold_uncertainty_score":0.9991121,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.08840060886553935,"score_gpt":0.4354907267280429,"score_spread":0.3470901178625035,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}