{"id":"W4416648334","doi":"10.5256/f1000research.171489.r325289","title":"Referee report. For: A guide to selecting high-performing antibodies for Rab10 (UniProt ID: P61026) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved]","year":2024,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Mitacs; Government of Canada; Ontario Genomics; Genome Canada","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Monoclonal antibody","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch"],"consensus_categories":[],"category_scores_codex":[0.007631862,0.0002575763,0.0006640733,0.0006144335,0.0003787291,0.0001471891,0.0003048108,0.0001440486,0.00002397767],"category_scores_gemma":[0.009246914,0.0002053873,0.0001860963,0.000657227,0.0001938954,0.0003679413,0.0002879323,0.0005285047,0.00001349197],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00023331,"about_ca_system_score_gemma":0.0004520412,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002461782,"about_ca_topic_score_gemma":0.0006029483,"domain_scores_codex":[0.9958922,0.0001246078,0.0008644854,0.0007282588,0.001533092,0.0008573104],"domain_scores_gemma":[0.9919025,0.001932705,0.0001073185,0.0004322437,0.005412846,0.0002124027],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.006165701,0.0002969466,0.01397429,0.03678865,0.0004486871,0.0001285131,0.002518722,0.000009723472,0.222017,0.0008720008,0.6558006,0.06097922],"study_design_scores_gemma":[0.001593709,0.001780539,0.007124791,0.00976608,0.00006980199,0.0001496502,0.0006325259,0.001580182,0.02285547,0.0002409851,0.9539164,0.0002898059],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"empirical","genre_gemma":"other","genre_scores_codex":[0.8445942,0.0171248,0.004805798,0.1156993,0.0002908276,0.01451411,0.002387373,0.0001261492,0.0004574087],"genre_scores_gemma":[0.1545524,0.01330402,0.1529879,0.0007348706,0.000938216,0.004682023,0.01082321,0.0003051787,0.6616722],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.6900418,"threshold_uncertainty_score":0.9990986,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07722091734473524,"score_gpt":0.4304931353948789,"score_spread":0.3532722180501436,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}