{"id":"W4416648334","doi":"10.5256/f1000research.171489.r325289","title":"Referee report. For: A guide to selecting high-performing antibodies for Rab10 (UniProt ID: P61026) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved]","year":2024,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Mitacs; Government of Canada; Ontario Genomics; Genome Canada","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Monoclonal antibody","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["metaresearch","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.007588311,0.001332383,0.001696163,0.006380973,0.003074048,0.004098212,0.003723328,0.003723641,0.7551999],"category_scores_gemma":[0.07069557,0.0009689581,0.001534716,0.004898788,0.0008663281,0.003277492,0.004978987,0.003124614,0.6445081],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002251373,"about_ca_system_score_gemma":0.006054769,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01305463,"about_ca_topic_score_gemma":0.02715471,"domain_scores_codex":[0.9946873,0.0008884085,0.0007705763,0.0006176963,0.002660112,0.0003757902],"domain_scores_gemma":[0.9315699,0.008106994,0.001356166,0.005353201,0.05058258,0.003031228],"domain_codex":null,"domain_gemma":"evaluation","domain_candidate":"evaluation","domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000008389872,0.00000869136,0.00003039954,0.0001010867,0.000001902604,0.00002139769,0.00001600926,0.000007030106,0.0001769413,0.0001460204,0.9914159,0.008066297],"study_design_scores_gemma":[0.00001283973,0.000009312923,0.0003920323,0.0001414009,0.00000618467,0.00008754186,0.00007129263,0.00002489818,0.0002638443,0.0006363891,0.9983343,0.00001998291],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"editorial","genre_gemma":"commentary","genre_scores_codex":[0.001192442,0.00684971,0.02879538,0.1353416,0.2968452,0.004362056,0.2325722,0.02340441,0.2706371],"genre_scores_gemma":[0.003507752,0.00494405,0.02158303,0.0250109,0.02472985,0.002700528,0.07881227,0.01009922,0.8286124],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.9924117,"threshold_uncertainty_score":0.3491776,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07722091734473524,"score_gpt":0.4304931353948789,"score_spread":0.3532722180501436,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}