{"id":"W4416653166","doi":"10.5256/f1000research.176085.r359970","title":"Referee report. For: A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved, 1 approved with reservations]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Genentech; Mitacs; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Ontario Genomics; Genome Canada; ALS Society of Canada; Bayer; Motor Neurone Disease Association; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Virus","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["metaresearch","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.01045607,0.001388553,0.001903244,0.007144555,0.003658843,0.006139043,0.004160965,0.003777578,0.755927],"category_scores_gemma":[0.1010119,0.001149942,0.001418326,0.006258267,0.001104164,0.004641551,0.006133439,0.003205157,0.7165775],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002244937,"about_ca_system_score_gemma":0.008124767,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01252142,"about_ca_topic_score_gemma":0.02674179,"domain_scores_codex":[0.9918972,0.001473727,0.001203554,0.0009106596,0.004009503,0.0005052193],"domain_scores_gemma":[0.8851908,0.01134827,0.002120389,0.009593966,0.08698201,0.00476458],"domain_codex":null,"domain_gemma":"evaluation","domain_candidate":"evaluation","domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000008274797,0.00000707768,0.00002471051,0.0001209978,0.000002014211,0.00001709821,0.00001829466,0.000006017408,0.0001604646,0.0001565946,0.9926146,0.006863736],"study_design_scores_gemma":[0.00001336812,0.000007528668,0.0002809197,0.000149308,0.000005856208,0.00006368149,0.00006427791,0.00002220972,0.0002124,0.0006170215,0.9985455,0.00001788931],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"dataset","genre_gemma":"commentary","genre_scores_codex":[0.001132296,0.006919345,0.03005644,0.120679,0.2684481,0.004389897,0.2695855,0.02938158,0.2694079],"genre_scores_gemma":[0.003187012,0.005026377,0.0202921,0.01857521,0.02097928,0.002713727,0.1086733,0.01449328,0.8060597],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.9895439,"threshold_uncertainty_score":0.3481405,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09957160105712172,"score_gpt":0.4261414308504364,"score_spread":0.3265698297933147,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}