{"id":"W4416654970","doi":"10.5256/f1000research.176020.r358321","title":"Referee report. For: A guide to selecting high-performing antibodies for STXBP1 (UniProt ID: P61764) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Genentech; Québec Consortium for Drug Discovery; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Simons Foundation Autism Research Initiative; Bayer; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Virus; Monoclonal antibody","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaresearch","metaepi_narrow"],"consensus_categories":["metaresearch"],"category_scores_codex":[0.03822582,0.001165919,0.003313687,0.002219385,0.001052417,0.0004048492,0.002084758,0.0007159528,0.00004532697],"category_scores_gemma":[0.09729086,0.001117173,0.0006204125,0.002375859,0.0003851045,0.001055445,0.001503539,0.001731331,0.00005630704],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001535787,"about_ca_system_score_gemma":0.002329522,"about_ca_topic_candidate":true,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.007704467,"about_ca_topic_score_gemma":0.005849627,"domain_scores_codex":[0.9859681,0.0009110246,0.003640301,0.002584694,0.004608175,0.002287677],"domain_scores_gemma":[0.9507312,0.004808031,0.001768027,0.002373579,0.03997725,0.0003419203],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0008923062,0.0001650483,0.0002937709,0.08907542,0.000338793,0.00001160369,0.0003526,0.000006743078,0.005469854,0.00003779702,0.8927193,0.01063672],"study_design_scores_gemma":[0.002025054,0.000827135,0.0003980768,0.1159059,0.000385084,0.00003998708,0.0003317295,0.0001288227,0.002003816,0.00008680198,0.8769537,0.0009138221],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"commentary","genre_gemma":"other","genre_scores_codex":[0.006679319,0.3263921,0.007342904,0.3541139,0.002457905,0.1531082,0.1474318,0.0007501655,0.001723681],"genre_scores_gemma":[0.00008636525,0.03717242,0.07568795,0.0005481031,0.0003282913,0.01276947,0.07108463,0.0004964258,0.8018264],"genre_candidate":"other","genre_consensus":null,"teacher_disagreement_score":0.8001027,"threshold_uncertainty_score":0.9991279,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.09688366611152889,"score_gpt":0.4421034357858395,"score_spread":0.3452197696743107,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}