{"id":"W4416658596","doi":"10.5256/f1000research.175953.r352666","title":"Referee report. For: A guide to selecting high-performing antibodies for ADNP (UniProt ID: Q9H2P0) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 1 approved]","year":2025,"lang":"en","type":"peer-review","venue":"Faculty of 1000 Research Ltd","topic":"","field":"","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Genentech; Government of Canada; Ontario Genomics Institute; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Simons Foundation Autism Research Initiative; Bayer; Pfizer; Bristol-Myers Squibb","keywords":"Immunofluorescence; Antibody; Indirect immunofluorescence; Virus; Monoclonal antibody","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.01021405,0.00125489,0.001650395,0.006699911,0.003370126,0.005137727,0.00359864,0.003537241,0.7369395],"category_scores_gemma":[0.09459394,0.0009931864,0.001283101,0.005109886,0.001035713,0.003872711,0.005184258,0.003248831,0.6516756],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.002379726,"about_ca_system_score_gemma":0.00801663,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.01382387,"about_ca_topic_score_gemma":0.02980039,"domain_scores_codex":[0.9923734,0.001426252,0.001078081,0.0008410439,0.003805536,0.0004757542],"domain_scores_gemma":[0.8867774,0.01202506,0.001967659,0.008141865,0.08642298,0.004665031],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000007917746,0.000008087033,0.00002776189,0.0001044835,0.000001743608,0.00001816333,0.00001851617,0.000006329873,0.0001412896,0.0001512827,0.9918412,0.007673108],"study_design_scores_gemma":[0.00001364967,0.000008516578,0.0003312934,0.0001463502,0.000005665262,0.00007029145,0.00007411072,0.00002426434,0.0002187288,0.0006245396,0.9984642,0.00001832264],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"other","genre_gemma":"commentary","genre_scores_codex":[0.001201214,0.006882613,0.02901547,0.1529647,0.2786065,0.005358658,0.2188666,0.02572251,0.2813818],"genre_scores_gemma":[0.003312735,0.004740112,0.02032546,0.02205848,0.0223643,0.002946607,0.07361475,0.01076128,0.8398763],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.7369395,"threshold_uncertainty_score":0.3752238,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0961578777933286,"score_gpt":0.4411159861521721,"score_spread":0.3449581083588434,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}