{"id":"W4416660354","doi":"10.5256/f1000research.156691.r228353","title":"Referee report. For: Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation [version 3; peer review: 1 approved, 2 approved with reservations]","year":2023,"lang":"en","type":"article","venue":"Faculty of 1000 Research Ltd","topic":"Atherosclerosis and Cardiovascular Diseases","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Genentech; Government of Canada; Ontario Genomics Institute; Emory University; European Federation of Pharmaceutical Industries and Associations; Merck KGaA; Mitacs; Ontario Genomics; Genome Canada; Bristol-Myers Squibb; Bayer; Pfizer","keywords":"Immunoprecipitation; Antibody; Identification (biology); Western blot; Blot; Apolipoprotein E","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":["metaresearch","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.006922125,0.0009341127,0.001530943,0.003419807,0.003646098,0.003179685,0.00265655,0.005967563,0.5294026],"category_scores_gemma":[0.08310736,0.0007029638,0.001433455,0.002448866,0.0008545072,0.002141394,0.00271065,0.004339641,0.3786908],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.003110305,"about_ca_system_score_gemma":0.00468654,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.02540888,"about_ca_topic_score_gemma":0.03710448,"domain_scores_codex":[0.994786,0.0007403698,0.0006649718,0.0006436731,0.00278815,0.000376843],"domain_scores_gemma":[0.8994738,0.01366822,0.001636379,0.005455022,0.07620952,0.003557028],"domain_codex":null,"domain_gemma":"evaluation","domain_candidate":"evaluation","domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.00001150668,0.00001429485,0.00004082887,0.00004255807,0.000002318784,0.00003454216,0.00001474962,0.000005778656,0.0001920852,0.0001523665,0.9962942,0.003194689],"study_design_scores_gemma":[0.00002929498,0.00002142999,0.001423604,0.0000958878,0.00001120414,0.0001613025,0.0001179125,0.00007719142,0.0005691538,0.0009593774,0.9964909,0.00004275256],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"editorial","genre_gemma":"commentary","genre_scores_codex":[0.001337023,0.003194464,0.009739265,0.2939222,0.4751638,0.002905127,0.06007414,0.009815328,0.1438487],"genre_scores_gemma":[0.006231497,0.002278953,0.007772964,0.06932592,0.04601229,0.001640943,0.02237842,0.003358292,0.8410007],"genre_candidate":"commentary","genre_consensus":null,"teacher_disagreement_score":0.9930779,"threshold_uncertainty_score":0.67125,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07845826487662061,"score_gpt":0.3569641237009238,"score_spread":0.2785058588243032,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}