{"id":"W4416834926","doi":"10.18331/brj2025.12.4.3","title":"Targeted silencing of exopolysaccharide biosynthesis genes via CRISPR/dCas9 to enhance polyhydroxybutyrate production from biodiesel-derived crude glycerol in Burkholderia sp.","year":2025,"lang":"en","type":"article","venue":"Biofuel Research Journal","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Thailand Science Research and Innovation; Chulalongkorn University; Chungbuk National University","keywords":"Polyhydroxybutyrate; Burkholderia; Gene silencing; Mutant; RNA interference; Biosynthesis; Glycerol; Bifunctional","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000187791,0.0004496001,0.0003354296,0.0001753184,0.0001043949,0.000356281,0.0002675581,0.0002976754,0.0005685544],"category_scores_gemma":[0.0001667063,0.0001350897,0.0002374025,0.0001627046,0.0002188334,0.0001589481,0.000355531,0.0004756618,0.000250488],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002709254,"about_ca_system_score_gemma":0.000293485,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001130493,"about_ca_topic_score_gemma":0.00181342,"domain_scores_codex":[0.9998026,0.00002629649,0.000030739,0.00004456297,0.0000607155,0.00003504667],"domain_scores_gemma":[0.9998907,0.0000191838,0.0000369921,0.00001352654,0.00001616983,0.0000234642],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001391953,0.00001014405,0.00005677407,0.00001481177,0.000001992238,0.00002418789,0.000005747314,0.00006971027,0.9992024,0.00003115627,0.000006353372,0.0005627987],"study_design_scores_gemma":[0.00000497165,0.00005950494,0.0006768269,0.000003126306,0.000007247339,0.00006466892,0.00002113509,0.001508802,0.996739,0.00001872556,0.000892323,0.000003670601],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9812272,0.0005465931,0.015965,0.0001197977,0.0000529513,0.00007029761,0.0004281511,0.0001969871,0.001393012],"genre_scores_gemma":[0.9871982,0.0003422736,0.009721363,0.00004795561,0.000003866769,0.00004379962,0.0003653007,0.00003550718,0.002241691],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001130493,"threshold_uncertainty_score":0.00224787,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04506153182750003,"score_gpt":0.337214475131196,"score_spread":0.2921529433036959,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}