{"id":"W4416956232","doi":"10.1016/j.crmeth.2025.101248","title":"FAME-CRISPR improves CRISPR-Cas9 genome editing via HDAC inhibition and engineered virus-like particle delivery","year":2025,"lang":"en","type":"article","venue":"Cell Reports Methods","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Alberta Children's Hospital; University of Calgary","funders":"Canadian Institutes of Health Research; Cumming School of Medicine, University of Calgary","keywords":"Genome editing; Histone deacetylase; CRISPR; Population; Transduction (biophysics); HDAC1; Gene; HDAC6; Selection (genetic algorithm); Histone","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007565694,0.0006119445,0.0006708647,0.0005445869,0.0002855599,0.0006768745,0.0007510745,0.0007700741,0.00256083],"category_scores_gemma":[0.0004756319,0.0004390226,0.0006124125,0.0002758006,0.0004044161,0.0003962089,0.0007095361,0.001176958,0.001235744],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005675544,"about_ca_system_score_gemma":0.000390098,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000886542,"about_ca_topic_score_gemma":0.002006411,"domain_scores_codex":[0.9991891,0.0001050002,0.00009043291,0.0002407943,0.0002730803,0.0001015481],"domain_scores_gemma":[0.9997068,0.00006531771,0.00007328323,0.00006347169,0.00004200872,0.00004913228],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004272055,0.00002453092,0.0001505657,0.00005211279,0.00001115238,0.00005066881,0.00001331297,0.0002569908,0.9947692,0.0003191796,0.0001825004,0.004127158],"study_design_scores_gemma":[0.000008759686,0.00007736935,0.0005811432,0.000004890299,0.00001333373,0.0002630559,0.000004201994,0.001954321,0.9897807,0.00005797411,0.007242245,0.00001205091],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.508535,0.004696653,0.4622369,0.0005059604,0.000415118,0.0006535536,0.003821601,0.008263676,0.01087145],"genre_scores_gemma":[0.7595697,0.001781601,0.2147329,0.0002176339,0.00004676217,0.0003560895,0.004114246,0.0006727061,0.01850837],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00256083,"threshold_uncertainty_score":0.008566797,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007160042193896066,"score_gpt":0.312678263287829,"score_spread":0.305518221093933,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}