{"id":"W4417132935","doi":"10.64898/2025.12.06.692781","title":"Conjugation Based CRISPR Antifungals (COBRA)","year":2025,"lang":"","type":"article","venue":"bioRxiv (Cold Spring Harbor Laboratory)","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"CRISPR; Electroporation; Plasmid; Yeast; Cas9; Gene; Genome editing","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002833804,0.0008814815,0.0006123625,0.0008725276,0.0002318318,0.0006419062,0.0004550414,0.0005706849,0.01857786],"category_scores_gemma":[0.0002947328,0.0003090202,0.0004541469,0.000641768,0.000239991,0.0003522987,0.0004955803,0.001364312,0.006983264],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003611851,"about_ca_system_score_gemma":0.000327461,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004312673,"about_ca_topic_score_gemma":0.0007483784,"domain_scores_codex":[0.9996026,0.0000409741,0.00002161526,0.0001087074,0.0001628,0.00006341861],"domain_scores_gemma":[0.9998844,0.00001856301,0.00003398679,0.0000208616,0.00001913057,0.00002317974],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002151705,0.0000875016,0.0004080595,0.0008439459,0.00007359567,0.0003622226,0.0000391914,0.001377431,0.9179979,0.003796408,0.01512036,0.0596781],"study_design_scores_gemma":[0.00004386242,0.0002101518,0.0007378811,0.00003561255,0.00003801584,0.0004119335,0.00001429278,0.00189513,0.9138681,0.0004352114,0.08228093,0.00002886928],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3744332,0.02416084,0.3998407,0.0047418,0.002781704,0.001796686,0.03724883,0.0416675,0.1133287],"genre_scores_gemma":[0.770563,0.01035919,0.1210938,0.0009424047,0.0001395109,0.0006374987,0.01431512,0.001033701,0.08091576],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.01857786,"threshold_uncertainty_score":0.06214911,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006274317567879451,"score_gpt":0.2517550132914194,"score_spread":0.24548069572354,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}