{"id":"W4417132990","doi":"10.1021/acssynbio.5c00628","title":"Construction and Functional Characterization of a Heterologous Quorum Sensing Circuit in <i>Clostridium sporogenes</i>","year":2025,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"Credit Valley Hospital; University of Waterloo","funders":"Mitacs","keywords":"Quorum sensing; Obligate; Heterologous; Heterologous expression; Synthetic biology; Reporter gene; Gene; Bacteria; Gene expression","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001441544,0.0001239767,0.0002007094,0.0001033468,0.00004558813,0.000008139377,0.00004069098,0.0002757892,0.000006414672],"category_scores_gemma":[0.0000512724,0.000122969,0.00003638686,0.0001055723,0.0002417616,0.000003637047,0.00007445217,0.00006755277,9.294977e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001030421,"about_ca_system_score_gemma":0.00005372461,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003320316,"about_ca_topic_score_gemma":0.00002314053,"domain_scores_codex":[0.9991042,0.00008963653,0.0002743105,0.0003121122,0.00002935196,0.0001904053],"domain_scores_gemma":[0.9996253,0.00001876428,0.0001081159,0.0001688235,0.00005445663,0.00002459962],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001087888,0.00001878408,0.008660491,0.0000153481,0.00002469851,9.125026e-7,0.000009629103,9.39719e-7,0.9690321,0.0007734236,0.000006583313,0.02134828],"study_design_scores_gemma":[0.0004274959,0.0001135812,0.01431003,0.000031304,0.00001785945,0.00008913819,0.00002523032,0.00001606184,0.9807736,0.0002241865,0.003856457,0.000115077],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972629,0.0001508177,0.001346437,0.0003687357,0.000525608,0.0001209288,0.00002772169,0.0000082928,0.0001885379],"genre_scores_gemma":[0.9990105,0.0001519206,0.0002520312,0.0002615698,0.00007159578,0.000002072815,0.0002087826,0.00000796473,0.00003356653],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0212332,"threshold_uncertainty_score":0.5014532,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00769121753514725,"score_gpt":0.2100323304584637,"score_spread":0.2023411129233164,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}