{"id":"W4417232957","doi":"10.1021/acs.analchem.5c05220","title":"Proteome-Wide Analysis and Surface Protein Isolation for Secretome Characterization Reveal Insights into the Biology of the Leaf-Cutter Ant <i>Acromyrmex echinatior</i>","year":2025,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Insect and Arachnid Ecology and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"American Society for Mass Spectrometry; National Institute of Diabetes and Digestive and Kidney Diseases; NIH Office of the Director; Secretaría Nacional de Ciencia, Tecnología e Innovación; National Institute on Aging; National Institutes of Health; National Institute of Food and Agriculture; Wisconsin Alumni Research Foundation; Division of Environmental Biology; University of Wisconsin-Madison; U.S. Department of Agriculture; National Center for Research Resources; Ministry of Environment; National Science Foundation","keywords":"Proteome; Proteomics; Secretory protein; ANT; Model organism; Protein subcellular localization prediction; Function (biology); Isolation (microbiology)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001142359,0.0003150901,0.0001841793,0.0003366364,0.0001802377,0.0003235688,0.00015671,0.0002480356,0.0004253699],"category_scores_gemma":[0.0001057136,0.0001217916,0.000241113,0.0002185532,0.0001278767,0.0002546614,0.0002722875,0.0004250955,0.0002079112],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001394974,"about_ca_system_score_gemma":0.0001149585,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005750207,"about_ca_topic_score_gemma":0.001705311,"domain_scores_codex":[0.9999053,0.000005409216,0.000007254238,0.00003708668,0.00003007659,0.00001489123],"domain_scores_gemma":[0.9999237,0.00001139585,0.00002222742,0.000008465583,0.00001829749,0.00001584789],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001331404,0.000002235558,0.0006701383,0.00001125456,0.000003349536,0.00001593272,0.00000868986,0.00001060041,0.9986381,0.000009267631,0.00001364421,0.0006034211],"study_design_scores_gemma":[0.000005953809,0.000109379,0.2302255,0.00001229879,0.00003630254,0.0005955484,0.000122987,0.002679513,0.7626889,0.00009077535,0.003422069,0.00001076931],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9883552,0.000797672,0.008166571,0.00008182482,0.00001245475,0.00003172013,0.001605283,0.0001038269,0.0008455009],"genre_scores_gemma":[0.9721313,0.0008969183,0.01935405,0.0001429217,0.00001283201,0.0000856914,0.004403956,0.00006359033,0.002908798],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005750207,"threshold_uncertainty_score":0.001423001,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004634063697093319,"score_gpt":0.2434433099895517,"score_spread":0.2388092462924584,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}