{"id":"W4417261578","doi":"10.1073/pnas.2536003123","title":"Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells","year":2025,"lang":"en","type":"preprint","venue":"Proceedings of the National Academy of Sciences","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Diabetes and Digestive and Kidney Diseases; Defense Sciences Office, DARPA; National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; National Institute of Environmental Health Sciences; Defense Advanced Research Projects Agency; National Science Foundation; National Cancer Institute; Damon Runyon Cancer Research Foundation","keywords":"Directed evolution; Directed Molecular Evolution; CRISPR; Cas9; Function (biology); Mutation; Adaptive evolution; Key (lock); Experimental evolution","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006997035,0.0002427646,0.0003691452,0.0002373778,0.000191539,0.0008013739,0.000498938,0.0005376506,0.002096453],"category_scores_gemma":[0.000437816,0.0001996865,0.000313824,0.0002104677,0.0003748919,0.0001774505,0.0005681355,0.0007888291,0.001009698],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004330998,"about_ca_system_score_gemma":0.0006293551,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009682734,"about_ca_topic_score_gemma":0.0008342655,"domain_scores_codex":[0.9994079,0.00009056854,0.00005304019,0.0001816893,0.0002092483,0.00005762924],"domain_scores_gemma":[0.9997681,0.00005277025,0.00004010057,0.00006184394,0.00004631624,0.00003090185],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000164823,0.00005771801,0.000966705,0.0001002999,0.00002803369,0.0001965461,0.00006545792,0.001314388,0.9773232,0.003159574,0.001035408,0.01558782],"study_design_scores_gemma":[0.00003924487,0.0003023216,0.00154492,0.00002612452,0.00003138155,0.000699487,0.00002873575,0.008639979,0.9445527,0.0004646471,0.04364735,0.00002295932],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6645798,0.003796858,0.2998586,0.0007680343,0.0004848715,0.0008171169,0.003371818,0.002938716,0.0233842],"genre_scores_gemma":[0.9147854,0.001093415,0.06811137,0.0003696551,0.00003519121,0.0002903316,0.002628402,0.0001627944,0.01252331],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002096453,"threshold_uncertainty_score":0.007013321,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01535512309848799,"score_gpt":0.3249431946526954,"score_spread":0.3095880715542074,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}