{"id":"W4417278213","doi":"10.1038/s41467-025-67201-w","title":"Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation","year":2025,"lang":"en","type":"article","venue":"Nature Communications","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hôpital Maisonneuve-Rosemont","funders":"Genentech","keywords":"CRISPR; Cas9; Genome editing; Nuclease; Genome engineering; Context (archaeology); Synthetic biology; Systems biology; DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003213014,0.0004779395,0.0003629113,0.0004142897,0.0002466429,0.0006533861,0.0008933222,0.0004667882,0.002055877],"category_scores_gemma":[0.0003029188,0.0002956565,0.0003280581,0.0002651845,0.0004712208,0.0004602072,0.0006607812,0.001188044,0.001034228],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006159971,"about_ca_system_score_gemma":0.0003472081,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004457524,"about_ca_topic_score_gemma":0.001079568,"domain_scores_codex":[0.9994794,0.00004620582,0.00003845457,0.0001337001,0.0002518871,0.00005045713],"domain_scores_gemma":[0.9997327,0.0000432433,0.00008388906,0.0000550246,0.00003740583,0.00004767017],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001736773,0.00001432715,0.00006081487,0.00005734408,0.000005651708,0.0000299336,0.000008654245,0.0003377196,0.9924855,0.001137309,0.000308294,0.005537067],"study_design_scores_gemma":[0.000006136692,0.00004650665,0.0003546532,0.000005201407,0.000009409149,0.0001356593,0.000004649292,0.002520626,0.98612,0.0002391658,0.01054738,0.0000106298],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2897106,0.003551257,0.6820272,0.0006537542,0.0004362827,0.0004527335,0.002104025,0.007332157,0.01373199],"genre_scores_gemma":[0.742792,0.002900504,0.235525,0.0004446282,0.00007803925,0.0004874417,0.002634369,0.0007609359,0.01437716],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.002055877,"threshold_uncertainty_score":0.006877601,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009031890699239944,"score_gpt":0.3289548614425922,"score_spread":0.3199229707433523,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}