{"id":"W4417410436","doi":"10.1016/j.gene.2025.149965","title":"In vitro expression and seroreactivity of Toxoplasma gondii GRA1 protein encoded by unmodified and nucleoside-modified mRNA constructs","year":2025,"lang":"en","type":"article","venue":"Gene","topic":"Toxoplasma gondii Research Studies","field":"Immunology and Microbiology","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Saskatchewan","funders":"Ege Üniversitesi","keywords":"Toxoplasma gondii; Messenger RNA; Immunogenicity; Recombinant DNA; Antibody; Western blot; Toxoplasmosis; Immune system","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003179992,0.0005423473,0.0002974958,0.0002015871,0.0002205458,0.0003318074,0.000245228,0.0002821626,0.001160005],"category_scores_gemma":[0.0002147042,0.000181156,0.0003745397,0.0002429413,0.000306366,0.0001953267,0.0001893089,0.0009071096,0.0004335765],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003616883,"about_ca_system_score_gemma":0.0001970305,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000595556,"about_ca_topic_score_gemma":0.0008678437,"domain_scores_codex":[0.9997931,0.00004584812,0.00002093215,0.00003723493,0.00005492155,0.00004792779],"domain_scores_gemma":[0.999742,0.0001126103,0.000051832,0.00003123991,0.00003094431,0.00003129179],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007593595,0.00002329301,0.0000652979,0.00002231312,0.000002504762,0.00002368843,0.0000234085,0.0000616061,0.9993911,0.00010039,0.00001323157,0.0001972738],"study_design_scores_gemma":[0.000008343702,0.0002036102,0.0009670893,0.000003765786,0.0000113394,0.00009088225,0.0000182342,0.0005468563,0.9972216,0.00002690869,0.0008975639,0.000003819879],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9923068,0.0006862952,0.00481942,0.00007599823,0.0000563355,0.00003348544,0.0007210742,0.00006517562,0.00123557],"genre_scores_gemma":[0.9794153,0.000827015,0.009815373,0.00007769869,0.00003754845,0.00005283619,0.004641594,0.00006978022,0.005062734],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001160005,"threshold_uncertainty_score":0.00388056,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008228974985216528,"score_gpt":0.2499430592625104,"score_spread":0.2417140842772939,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}