{"id":"W582856694","doi":"10.3791/52807","title":"Transfecting RAW264.7 Cells with a Luciferase Reporter Gene","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity; University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Transfection; Luciferase; Reporter gene; Cell culture; Cell biology; Plasmid; Molecular biology; Cell; Biology; Chemistry; Gene; Gene expression; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001491615,0.001167454,0.00113971,0.001213581,0.0006349028,0.0007156323,0.001213004,0.0009781912,0.006051837],"category_scores_gemma":[0.0003525621,0.0004547813,0.001080973,0.001445248,0.0005267893,0.0004942481,0.0005590114,0.001971671,0.008234438],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004290993,"about_ca_system_score_gemma":0.0005926117,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005935288,"about_ca_topic_score_gemma":0.001469726,"domain_scores_codex":[0.9988218,0.0001931602,0.00012773,0.0002361379,0.0004505072,0.0001705468],"domain_scores_gemma":[0.9996998,0.00005860576,0.00004570956,0.00007094139,0.0000823708,0.00004258284],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006190206,0.00007680142,0.0001000463,0.0002510529,0.0000166206,0.00007644031,0.00003450137,0.00005434187,0.9952883,0.0004792706,0.001098605,0.002462215],"study_design_scores_gemma":[0.00004067282,0.0005148212,0.001348384,0.00003449791,0.00005353954,0.0005467902,0.00004917358,0.001006351,0.9391271,0.0002974154,0.05693862,0.00004273188],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3427898,0.01776863,0.5483028,0.002097479,0.003162444,0.003511668,0.03241637,0.009003988,0.04094687],"genre_scores_gemma":[0.3151818,0.02467654,0.5067424,0.002047128,0.0007221377,0.006015792,0.06214891,0.001538512,0.08092691],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006051837,"threshold_uncertainty_score":0.02024543,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03520286367969758,"score_gpt":0.3894917950982565,"score_spread":0.3542889314185589,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}