{"id":"W582856694","doi":"10.3791/52807","title":"Transfecting RAW264.7 Cells with a Luciferase Reporter Gene","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute of Infection and Immunity; University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Transfection; Luciferase; Reporter gene; Cell culture; Cell biology; Plasmid; Molecular biology; Cell; Biology; Chemistry; Gene; Gene expression; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003209836,0.0001485681,0.0002253205,0.00006368311,0.00003344948,0.00003504931,0.0001519534,0.0000815345,0.00002486787],"category_scores_gemma":[0.00002740018,0.0001105276,0.0001207518,0.00005924259,0.00003316242,0.00001331217,0.00002817787,0.0000795705,0.000006107282],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002517766,"about_ca_system_score_gemma":0.0001512085,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009866465,"about_ca_topic_score_gemma":0.000001867306,"domain_scores_codex":[0.9988759,0.00007202756,0.0004212261,0.0001718249,0.0002746278,0.0001844521],"domain_scores_gemma":[0.999047,0.000004634918,0.0002793111,0.0001855779,0.0002908543,0.000192562],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001174613,0.0001667833,0.0006382164,0.000003936217,0.0001681702,0.0001328912,0.0005041321,0.0000521581,0.9935721,9.711038e-7,0.003255406,0.0003306154],"study_design_scores_gemma":[0.002498709,0.001985472,0.00003294204,0.0000344684,0.00003326991,0.0004590102,0.0005503627,0.00001409765,0.9896978,0.000007407441,0.004540041,0.0001464595],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.992383,0.0009135353,0.005745383,0.00002470271,0.0003329794,0.00008646532,0.000001936397,0.000004149668,0.00050786],"genre_scores_gemma":[0.9962304,0.00007819026,0.00269564,0.0002622074,0.0003274977,0.000004821369,0.000007303196,0.0000225917,0.0003713558],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003874346,"threshold_uncertainty_score":0.4507183,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03520286367969758,"score_gpt":0.3894917950982565,"score_spread":0.3542889314185589,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}