{"id":"W593452914","doi":"10.3791/52799","title":"Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution &lt;em&gt;In Situ&lt;/em&gt; Hybridization","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":true,"ca_institutions":"Columbia College","funders":"National Institute of Neurological Disorders and Stroke; National Institute of Mental Health; National Institute on Aging; Alzheimer's Association","keywords":"In situ hybridization; Biology; Transcriptome; Cell biology; Axon guidance; Messenger RNA; Axon; Translation (biology); In vivo; Neurodegeneration; Molecular biology; Computational biology; Gene expression; Pathology; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001048305,0.0001676036,0.0003347692,0.0004241868,0.00005823338,0.00003889213,0.0001662373,0.000164116,0.00000944955],"category_scores_gemma":[0.0004613097,0.0001654235,0.0001272655,0.0003737047,0.00004555375,0.00004423517,0.0000752667,0.0001702067,0.000001906794],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002740245,"about_ca_system_score_gemma":0.0002699029,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001376298,"about_ca_topic_score_gemma":0.0003513346,"domain_scores_codex":[0.9977809,0.0003706072,0.0007698684,0.0002186911,0.0005522546,0.0003076972],"domain_scores_gemma":[0.9987974,0.00002145771,0.0004512574,0.000170131,0.0003823446,0.0001774065],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001336675,0.0002995581,0.0005291249,0.00001383764,0.00006057799,0.00001655848,0.0002717619,0.004041405,0.9926776,0.00002949294,0.000168034,0.0005554167],"study_design_scores_gemma":[0.005803115,0.0008384771,0.001546521,0.0001182128,0.00001436511,0.00007307143,0.0004765016,0.006568077,0.9835461,0.00008685781,0.0007646544,0.0001640232],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9618884,0.001119607,0.03643412,0.00003809679,0.0002604575,0.0001946082,0.000003302766,0.000004696861,0.0000566606],"genre_scores_gemma":[0.9972131,0.00009907908,0.002282836,0.00005428547,0.0001840255,0.000007834326,0.0000203741,0.00002538973,0.0001130218],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0353247,"threshold_uncertainty_score":0.6745773,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0313464274081923,"score_gpt":0.3733416090199738,"score_spread":0.3419951816117816,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}