{"id":"W67224284","doi":"10.2144/00283st10","title":"Polycationic Lipids Translocate Lipopolysaccharide into HeLa Cells","year":2000,"lang":"en","type":"article","venue":"BioTechniques","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Faculty of Medicine, University of British Columbia; Heart and Stroke Foundation of Canada","keywords":"HeLa; Lipofectamine; Lipopolysaccharide; Molecular biology; Transfection; Viability assay; Biology; Fluorescein isothiocyanate; Cell culture; Phorbol; Cytoplasm; Cell; Chemistry; Cell biology; Biochemistry; Immunology; Fluorescence; Protein kinase C; Signal transduction; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003189307,0.0004247526,0.0004165957,0.0002444152,0.0002810283,0.0005513944,0.0002997798,0.0005242342,0.001190596],"category_scores_gemma":[0.0002337448,0.0002079457,0.0003542636,0.0002275796,0.0002719642,0.0003808545,0.0003529182,0.0007050239,0.001197447],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003859468,"about_ca_system_score_gemma":0.0002418514,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006919766,"about_ca_topic_score_gemma":0.001013308,"domain_scores_codex":[0.9996731,0.00006093664,0.00003781182,0.0000815844,0.0000969126,0.00004971388],"domain_scores_gemma":[0.9998484,0.00004955313,0.00002404503,0.00002392492,0.00003777479,0.00001626247],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002565787,0.000008413673,0.00002870538,0.00005058855,0.000002618918,0.00002728449,0.00004114134,0.00003062916,0.9990663,0.00006734319,0.0000366126,0.0006147902],"study_design_scores_gemma":[0.00001304517,0.0002316202,0.0005779201,0.0000126624,0.00001363827,0.0001144832,0.00003716974,0.0005882539,0.9912933,0.00004347468,0.007065546,0.000009016203],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9127058,0.008703233,0.06246101,0.0008861864,0.0006041762,0.000467641,0.002712227,0.0007829738,0.01067669],"genre_scores_gemma":[0.9075446,0.006531507,0.06299253,0.0004924812,0.00009705659,0.0004413014,0.004440031,0.0002438811,0.01721667],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001190596,"threshold_uncertainty_score":0.003982961,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006102273751806221,"score_gpt":0.2380280305621049,"score_spread":0.2319257568102986,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}