{"id":"W67224284","doi":"10.2144/00283st10","title":"Polycationic Lipids Translocate Lipopolysaccharide into HeLa Cells","year":2000,"lang":"en","type":"article","venue":"BioTechniques","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Faculty of Medicine, University of British Columbia; Heart and Stroke Foundation of Canada","keywords":"HeLa; Lipofectamine; Lipopolysaccharide; Molecular biology; Transfection; Viability assay; Biology; Fluorescein isothiocyanate; Cell culture; Phorbol; Cytoplasm; Cell; Chemistry; Cell biology; Biochemistry; Immunology; Fluorescence; Protein kinase C; Signal transduction; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001023821,0.0001549251,0.0001118788,0.00004690781,0.00007584018,0.00003384212,0.0002671938,0.0002513734,0.0002583622],"category_scores_gemma":[0.000004649872,0.0001493266,0.0001031495,0.00006171704,0.00007393161,0.000006362115,0.00002718661,0.00009420433,0.0001243718],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001297282,"about_ca_system_score_gemma":0.0000391324,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002479309,"about_ca_topic_score_gemma":0.00004858444,"domain_scores_codex":[0.9991591,0.00003460162,0.0001918801,0.000320695,0.00008735999,0.0002063678],"domain_scores_gemma":[0.9994727,0.000003205913,0.00003276908,0.0003848709,0.00004011367,0.0000663324],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004873109,0.00003472756,0.00002557991,0.000007386348,0.00001718668,0.000001102243,0.00004792183,0.00000289551,0.9407706,0.00002758392,0.006579325,0.05243698],"study_design_scores_gemma":[0.00008250731,0.000267301,0.00004289648,0.00001570144,0.000008995338,0.00000599573,0.0000128022,0.00001739848,0.8887681,0.0001690747,0.110433,0.0001761949],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9899085,0.001206793,0.005588359,0.0008510252,0.0001265908,0.0002142707,0.00001350806,0.0001324121,0.001958538],"genre_scores_gemma":[0.9896972,0.003021108,0.002738934,0.001112415,0.0002940137,0.00003799295,0.00005064043,0.00002340578,0.003024288],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1038537,"threshold_uncertainty_score":0.608936,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006102273751806221,"score_gpt":0.2380280305621049,"score_spread":0.2319257568102986,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}