{"id":"W6893198110","doi":"10.5281/zenodo.14528462","title":"A guide to selecting high-performing antibodies for DYRK1A (UniProt ID: Q13627) for use in western blot and immunoprecipitation","year":2024,"lang":"en","type":"article","venue":"Zenodo (CERN European Organization for Nuclear Research)","topic":"Down syndrome and intellectual disability research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Montreal Neurological Institute and Hospital","funders":"","keywords":"Immunoprecipitation; Gene knockdown; Western blot; Antibody; DYRK1A; Autism; Cell culture","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005085221,0.00488373,0.004193965,0.008533597,0.00348237,0.003124959,0.005263024,0.002553567,0.1423886],"category_scores_gemma":[0.008704009,0.005588761,0.002330468,0.004653067,0.001104457,0.002198558,0.002248451,0.004662026,0.1797549],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008320803,"about_ca_system_score_gemma":0.002375579,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001789116,"about_ca_topic_score_gemma":0.007280149,"domain_scores_codex":[0.9960419,0.0009440812,0.0007101911,0.0006410682,0.001197159,0.0004657125],"domain_scores_gemma":[0.9938699,0.002056235,0.000367227,0.001412031,0.001683348,0.0006111506],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"not_applicable","study_design_gemma":"not_applicable","study_design_scores_codex":[0.001365795,0.0005817257,0.001399634,0.002867526,0.0002358065,0.001249756,0.0002637877,0.0006444897,0.4373714,0.002738975,0.4720932,0.07918791],"study_design_scores_gemma":[0.0004773668,0.0006039508,0.006170558,0.000521023,0.0002014747,0.004872625,0.0001362461,0.001624328,0.09664127,0.003336774,0.8851663,0.0002480839],"study_design_candidate":"not_applicable","study_design_consensus":"not_applicable","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.01466267,0.01535981,0.6163655,0.005446512,0.002590858,0.01314206,0.2116032,0.06171432,0.05911506],"genre_scores_gemma":[0.008804136,0.01081038,0.6478624,0.002421337,0.0007203607,0.01821925,0.2507353,0.0151918,0.04523508],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.1423886,"threshold_uncertainty_score":0.4763372,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0601847824536475,"score_gpt":0.3303829405452662,"score_spread":0.2701981580916187,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}