{"id":"W6893230235","doi":"10.5281/zenodo.14367126","title":"A guide to selecting high-performing antibodies for Rab27A (UniProt ID: P51159) and Rab27B (UniProt ID: O00194) for use in western blot, immunoprecipitation, and immunofluorescence","year":2024,"lang":"en","type":"article","venue":"Zenodo (CERN European Organization for Nuclear Research)","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Montreal Neurological Institute and Hospital","funders":"","keywords":"Antibody; Immunofluorescence; Gene knockdown; Indirect immunofluorescence; Cell culture; Line (geometry)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003590926,0.004767308,0.003482474,0.008073997,0.002974135,0.002515184,0.004200965,0.001747512,0.1066436],"category_scores_gemma":[0.004998441,0.004257301,0.002041484,0.005516586,0.0009000268,0.001973069,0.001665512,0.00415098,0.1456597],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007921073,"about_ca_system_score_gemma":0.002187697,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001532967,"about_ca_topic_score_gemma":0.005421521,"domain_scores_codex":[0.9974951,0.0005626244,0.0004791906,0.0004444353,0.0007005951,0.0003181162],"domain_scores_gemma":[0.9961128,0.001147176,0.0002709218,0.001124504,0.0009550336,0.0003894984],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001065471,0.0005271106,0.00117749,0.003058542,0.0002122169,0.0008980496,0.0002464785,0.0006542434,0.5839819,0.002615136,0.3268864,0.07867696],"study_design_scores_gemma":[0.0002544495,0.000502708,0.005557585,0.0003866907,0.0001962797,0.00412753,0.0001105792,0.001752139,0.129866,0.002476473,0.8545536,0.0002159761],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.01754736,0.01501654,0.5838432,0.002547918,0.001723153,0.007424648,0.2618926,0.06769472,0.04230976],"genre_scores_gemma":[0.009192243,0.009304839,0.5793753,0.001399139,0.0004171074,0.009473094,0.3411544,0.01487492,0.03480887],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.1066436,"threshold_uncertainty_score":0.3567584,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01878286459253599,"score_gpt":0.2547303642197036,"score_spread":0.2359474996271677,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}