{"id":"W6903363607","doi":"10.1021/bi401166f.s001","title":"Kinetic and Structural Evaluation of Selected Active\\nSite Mutants of the <i>Aspergillus fumigatus</i> KDNase\\n(Sialidase)","year":2016,"lang":"en","type":"article","venue":"Figshare","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Directorate for Biological Sciences; Natural Sciences and Engineering Research Council of Canada; Scottish Funding Council; Wellcome Trust","keywords":"Mutant; Steric effects; Nucleophile; Substrate (aquarium); Catalysis; Active site; Neuraminidase; Enzyme; Binding site; Sialidase","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.00006526167,0.0001150201,0.0001128169,0.00003114097,0.00004949147,0.000008143117,0.0001125578,0.0001121902,0.004623751],"category_scores_gemma":[0.0008414051,0.0000754414,0.00004499695,0.0001487244,0.00003052784,0.00001066008,0.00008243787,0.00003425992,0.000007657476],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001762005,"about_ca_system_score_gemma":0.00009227257,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":6.510978e-7,"about_ca_topic_score_gemma":0.00001895781,"domain_scores_codex":[0.9990932,0.0001243169,0.0001857243,0.0002386675,0.0002380738,0.0001200281],"domain_scores_gemma":[0.9988755,0.00001197474,0.0002044906,0.0002670304,0.0006029663,0.00003804224],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004090914,0.00001174826,0.0001312946,0.0000356331,0.00002750194,1.07063e-7,0.00004475801,0.000006053723,0.9913173,0.000009218634,0.002046751,0.00632867],"study_design_scores_gemma":[0.0005601658,0.00006323362,0.1470547,0.0001689449,0.00002921631,0.000008228672,0.000004548103,0.0001078981,0.845331,0.000017079,0.00655826,0.00009666508],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9831269,0.0003262859,0.000003937574,0.00009866882,0.00008382942,0.0002682337,0.01588191,0.000008297829,0.0002019149],"genre_scores_gemma":[0.9917839,0.00001755566,0.00001494344,0.00002826851,0.0001211217,0.00002423434,0.007796851,0.00001419851,0.0001989856],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1469234,"threshold_uncertainty_score":0.9962862,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01591974406425685,"score_gpt":0.2448489401290869,"score_spread":0.2289291960648301,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}