{"id":"W6903363607","doi":"10.1021/bi401166f.s001","title":"Kinetic and Structural Evaluation of Selected Active\\nSite Mutants of the <i>Aspergillus fumigatus</i> KDNase\\n(Sialidase)","year":2016,"lang":"en","type":"article","venue":"Figshare","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Directorate for Biological Sciences; Natural Sciences and Engineering Research Council of Canada; Scottish Funding Council; Wellcome Trust","keywords":"Mutant; Steric effects; Nucleophile; Substrate (aquarium); Catalysis; Active site; Neuraminidase; Enzyme; Binding site; Sialidase","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003343261,0.0003911184,0.0003462323,0.0001473088,0.0001737195,0.0003742026,0.000449314,0.000315513,0.0008066703],"category_scores_gemma":[0.0004072453,0.0002085724,0.000352132,0.0002769119,0.0001952453,0.0002531392,0.0002391661,0.0005096555,0.0004720368],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00068734,"about_ca_system_score_gemma":0.0002898816,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004580082,"about_ca_topic_score_gemma":0.00471471,"domain_scores_codex":[0.9996694,0.00004893344,0.0000347092,0.00005926245,0.00012865,0.00005896198],"domain_scores_gemma":[0.9997576,0.00004845982,0.00004748917,0.00003109747,0.00007397278,0.00004144541],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002800106,0.0001834565,0.0007818492,0.00004118298,0.00001321561,0.00004636332,0.00005764002,0.0008332101,0.9953265,0.0001482384,0.0001136771,0.00217474],"study_design_scores_gemma":[0.00001144581,0.0002865148,0.002073601,0.000003613054,0.00001177357,0.00007360996,0.00004324766,0.003604891,0.9927984,0.00001948484,0.001063511,0.00000991301],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9966701,0.0003821942,0.00149859,0.00004805357,0.00002114415,0.0000194353,0.0004711672,0.0000324387,0.000856836],"genre_scores_gemma":[0.9932133,0.0002952669,0.002600781,0.00001778503,0.000003351528,0.00003119153,0.001340477,0.0000505236,0.002447385],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004580082,"threshold_uncertainty_score":0.009106815,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01591974406425685,"score_gpt":0.2448489401290869,"score_spread":0.2289291960648301,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}