{"id":"W6904534087","doi":"10.1371/journal.pone.0301562.s001","title":"In vitro validation of scRNA-seq protocol for nc886 detection.","year":2024,"lang":"en","type":"article","venue":"Figshare","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"","keywords":"Complementary DNA; Ethidium bromide; SYBR Green I; Primer (cosmetics); Agarose gel electrophoresis; Agarose; Real-time polymerase chain reaction; Rapid amplification of cDNA ends","routes":{"ca_aff":false,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002653409,0.002122466,0.002026467,0.001302605,0.001454526,0.001220712,0.002293072,0.0009927455,0.01225875],"category_scores_gemma":[0.002333417,0.001239615,0.001620484,0.001219629,0.0009424284,0.0005400849,0.001129998,0.00279594,0.02208795],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00101658,"about_ca_system_score_gemma":0.002008181,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003120588,"about_ca_topic_score_gemma":0.01194721,"domain_scores_codex":[0.9963182,0.0006011564,0.0003330153,0.00099542,0.001359486,0.0003927544],"domain_scores_gemma":[0.9980859,0.0003077512,0.0001410698,0.0005014171,0.0008060383,0.0001577558],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001439738,0.00007839842,0.0002182749,0.000531486,0.00003896795,0.0001690119,0.0001494122,0.0004068583,0.9823356,0.0005869268,0.008301291,0.007039798],"study_design_scores_gemma":[0.00008199487,0.0002120127,0.001943438,0.0001208107,0.0000978973,0.0007360488,0.00007851348,0.004559081,0.8633685,0.0005402117,0.1281651,0.00009634555],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05233943,0.004605258,0.8192149,0.0008672425,0.002060906,0.006132359,0.08001406,0.0132327,0.02153317],"genre_scores_gemma":[0.06338765,0.003745601,0.7138138,0.001816432,0.0002629228,0.01307604,0.1642025,0.005824128,0.03387093],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01225875,"threshold_uncertainty_score":0.04100955,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0282210701219301,"score_gpt":0.3476495004686834,"score_spread":0.3194284303467533,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}