{"id":"W6940644657","doi":"10.1021/bi100513u.s001","title":"Brønsted Analysis of an Enzyme-Catalyzed Pseudo-Deglycosylation Reaction: Mechanism of Desialylation in Sialidases","year":2016,"lang":"en","type":"article","venue":"Figshare","topic":"Mycorrhizal Fungi and Plant Interactions","field":"Agricultural and Biological Sciences","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"","keywords":"Sialidase; Glycosidic bond; Hydrolysis; Leaving group; Protonation; Kinetic isotope effect; Cleavage (geology); Mutant; Catalysis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002338619,0.000602664,0.0002116937,0.0001272262,0.00009454868,0.0002146105,0.00039766,0.0002556832,0.000951212],"category_scores_gemma":[0.000296886,0.0001895165,0.0002236926,0.0001647786,0.0001918357,0.0003318305,0.0001600009,0.000605759,0.0003010517],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00034294,"about_ca_system_score_gemma":0.0002093097,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001391302,"about_ca_topic_score_gemma":0.001118845,"domain_scores_codex":[0.9998397,0.00002802268,0.00001005279,0.00003510041,0.00005290495,0.00003425002],"domain_scores_gemma":[0.9998932,0.00002247174,0.00002742551,0.00001304514,0.00002244236,0.00002151185],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001020589,0.00001111569,0.0002292049,0.00003038596,0.00001102996,0.00005116223,0.00002345944,0.0002285688,0.9977838,0.0001962763,0.00002205879,0.00131085],"study_design_scores_gemma":[0.000001980985,0.0000494401,0.0006076297,0.000001557055,0.000003700688,0.00003745723,0.0000124471,0.001236133,0.9977885,0.00002949519,0.0002281964,0.000003373965],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9863678,0.0005695767,0.01122314,0.00004873318,0.00001431383,0.0000205642,0.0002394985,0.00006425889,0.001452052],"genre_scores_gemma":[0.9952154,0.0003085688,0.003202128,0.00001476921,0.000001517962,0.00001181112,0.0002812054,0.00001870964,0.0009458191],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001391302,"threshold_uncertainty_score":0.003182113,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03150734457465036,"score_gpt":0.2382175014067619,"score_spread":0.2067101568321116,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}