{"id":"W6959336637","doi":"10.6084/m9.figshare.c.7603182","title":"A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440","year":2024,"lang":"en","type":"other","venue":"Figshare","topic":"Date Palm Research Studies","field":"Agricultural and Biological Sciences","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"Pseudomonas putida; Genome editing; Recombineering; Plasmid; Genome; Transposable element; Electroporation; Bacterial genome size","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004360361,0.0008171035,0.0006191705,0.0005484931,0.000393457,0.0006377542,0.000832317,0.00060553,0.001343397],"category_scores_gemma":[0.0003834272,0.0004043814,0.0005176732,0.0002822673,0.0003033659,0.0003745704,0.0005776776,0.001036743,0.0009780579],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004822897,"about_ca_system_score_gemma":0.0005124855,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001354288,"about_ca_topic_score_gemma":0.001824813,"domain_scores_codex":[0.9993348,0.00006467368,0.00008513073,0.0001732328,0.0002714828,0.00007069036],"domain_scores_gemma":[0.9997166,0.00004163512,0.00007137466,0.00005733742,0.00004676897,0.00006634938],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002688526,0.00002331377,0.0001002637,0.00005959753,0.000007038265,0.0001092818,0.00001736658,0.0002262528,0.9954892,0.00009645307,0.0001350225,0.003709346],"study_design_scores_gemma":[0.00001032139,0.0001106135,0.0006688996,0.000007304908,0.00001795458,0.0003023981,0.00001648683,0.002775611,0.9926689,0.0000514006,0.003344857,0.00002519969],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6881459,0.001556549,0.2924888,0.0005952864,0.0003567458,0.000974067,0.003235566,0.007067419,0.005579576],"genre_scores_gemma":[0.8204746,0.000974471,0.1655952,0.0001174816,0.00002972659,0.0003992552,0.00297399,0.00041992,0.0090154],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001354288,"threshold_uncertainty_score":0.004494131,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06971863331668021,"score_gpt":0.2794728979829303,"score_spread":0.2097542646662501,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}