{"id":"W6963053490","doi":"10.18131/6gwpa-6zn57","title":"Probing the SAM Binding Site of SARS-CoV-2 Nsp14 In Vitro Using SAM Competitive Inhibitors Guides Developing Selective Bisubstrate Inhibitors","year":2021,"lang":"en","type":"article","venue":"Galter Health Sciences Library, Northwestern University","topic":"RNA modifications and cancer","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Institute of Allergy and Infectious Diseases; National Institute of General Medical Sciences","keywords":"Enzyme; Lysine; Non-competitive inhibition; Methylation; Methyltransferase; RNA; In vitro; Mechanism (biology); Binding site","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003655579,0.000650252,0.0005304337,0.0003440572,0.000139348,0.0002652005,0.0004355768,0.0003148528,0.001247729],"category_scores_gemma":[0.0002317905,0.0002643243,0.000286897,0.000327071,0.0001575406,0.0002295535,0.0002319333,0.0004457973,0.0004527775],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003287936,"about_ca_system_score_gemma":0.0002774447,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007002165,"about_ca_topic_score_gemma":0.002475734,"domain_scores_codex":[0.9998041,0.00005164633,0.0000187178,0.00003176802,0.00005989114,0.0000339116],"domain_scores_gemma":[0.9999046,0.00002701549,0.00001987849,0.000007875778,0.00002456562,0.00001600123],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001412385,0.0001263796,0.0003991651,0.0001412599,0.00001917124,0.00005771616,0.00002063166,0.0007895473,0.9909634,0.0001397669,0.0001146487,0.007087095],"study_design_scores_gemma":[0.00004966066,0.001571818,0.001185428,0.00001350521,0.00004022763,0.0001870399,0.00002688551,0.001957238,0.9896418,0.00005171965,0.005264801,0.000009756529],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9590307,0.0123407,0.01890014,0.0002385942,0.00005297881,0.0004135135,0.001093104,0.000222062,0.007708288],"genre_scores_gemma":[0.9620485,0.01136199,0.0194569,0.0001853256,0.00003168637,0.0002099136,0.002218831,0.00004166101,0.004445163],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001247729,"threshold_uncertainty_score":0.004174113,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04223396118478893,"score_gpt":0.2881003529469131,"score_spread":0.2458663917621241,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}