{"id":"W6968252748","doi":"10.5281/zenodo.15801995","title":"A guide to selecting high-performing antibodies for Rab8A (UniProt ID: P61006) for use in western blot, immunoprecipitation, and immunofluorescence","year":2025,"lang":"en","type":"article","venue":"Zenodo (CERN European Organization for Nuclear Research)","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Montreal Neurological Institute and Hospital","funders":"","keywords":"Immunofluorescence; Antibody; Protocol (science); Work (physics); Set (abstract data type)","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":true,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005723014,0.005152029,0.00435289,0.009060768,0.003752974,0.003214183,0.005778331,0.002304683,0.1069352],"category_scores_gemma":[0.007751978,0.006387422,0.002660026,0.005146811,0.00121043,0.002137466,0.002432787,0.005038403,0.1687907],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001032465,"about_ca_system_score_gemma":0.002612385,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002200072,"about_ca_topic_score_gemma":0.008502997,"domain_scores_codex":[0.9955884,0.001015493,0.0008163554,0.0006349639,0.001460012,0.0004847923],"domain_scores_gemma":[0.9935823,0.001872627,0.0003575789,0.001852672,0.001769556,0.0005652717],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.001255594,0.0006374072,0.001248136,0.002818887,0.0002412712,0.001361134,0.0003084829,0.0007511846,0.5514804,0.002946018,0.3422129,0.09473849],"study_design_scores_gemma":[0.0003840827,0.0006474091,0.005807139,0.0005214334,0.0001958999,0.004636131,0.0001203603,0.001711902,0.1198372,0.002844645,0.8630271,0.0002666822],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.01293982,0.01078432,0.7058536,0.003068242,0.001957432,0.01306113,0.1497865,0.06272017,0.03982881],"genre_scores_gemma":[0.007663491,0.009438281,0.7086186,0.001606451,0.0005460758,0.01736089,0.2009109,0.01518602,0.03866929],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.1069352,"threshold_uncertainty_score":0.3577337,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01566825233023692,"score_gpt":0.2560853966187245,"score_spread":0.2404171442884876,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}