{"id":"W6969163752","doi":"10.5281/zenodo.7462744","title":"Ligation Independent Cloning of transporter gene into BacMam vector","year":2022,"lang":"en","type":"article","venue":"Zenodo (CERN European Organization for Nuclear Research)","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Discovery Centre","funders":"Horizon 2020 Framework Programme","keywords":"Gene; Cloning (programming); Vector (molecular biology); clone (Java method); Transfection; Ligation; Molecular cloning","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001400311,0.001295608,0.001885078,0.001733807,0.0009179407,0.00112071,0.001552252,0.001459011,0.01083046],"category_scores_gemma":[0.0009263191,0.0009435298,0.001293915,0.001755053,0.0006878846,0.0006859665,0.001216035,0.003236594,0.0140316],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000507745,"about_ca_system_score_gemma":0.000707093,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007032017,"about_ca_topic_score_gemma":0.001110882,"domain_scores_codex":[0.9983634,0.0002717251,0.0002052592,0.0004678984,0.0004181524,0.0002735138],"domain_scores_gemma":[0.9995213,0.00009681502,0.00006867491,0.0001205585,0.0001080489,0.00008461906],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001567407,0.0001047869,0.0001920501,0.0005388582,0.00002936517,0.0002180945,0.0001239776,0.00007901813,0.9829617,0.001950005,0.002744389,0.01090092],"study_design_scores_gemma":[0.0001433571,0.000497355,0.002778422,0.0001225557,0.0001388048,0.0013452,0.00007870357,0.001716831,0.7596821,0.0008297827,0.2325859,0.00008087666],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2185545,0.01112376,0.6756144,0.001186592,0.002536636,0.008292409,0.02888921,0.01330863,0.04049387],"genre_scores_gemma":[0.2687722,0.009088428,0.4678162,0.0009078916,0.0004453286,0.00615343,0.1372293,0.00339626,0.1061909],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.01083046,"threshold_uncertainty_score":0.03623152,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01370248443829388,"score_gpt":0.2335888561682044,"score_spread":0.2198863717299105,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}